Wang Meng, Hu Rong, Yang Yuanyuan, Xiang Liping, Mu Yuming
Department of Echocardiography, First Affiliated Hospital, Xinjiang Medical University, Ürümqi, China.
Front Pharmacol. 2019 Aug 21;10:899. doi: 10.3389/fphar.2019.00899. eCollection 2019.
Stem cell therapy of acute myocardial infarction (AMI) is proving to be a promising approach to repair the injured myocardia. The time window for stem cell transplantation is crucial yet difficult to determine since it produces different therapeutic effects at different times after myocardial infarction. Stromal cell-derived factor-1 (SDF- 1) plays a pivotal role in the mobilization, homing, proliferation, and differentiation of transplanted stem cells. Here, by using ultrasound molecular imaging targeted microbubbles, we determined the dynamic expression of SDF-1 in a swine model of AMI . Twenty-four miniswine were randomly selected for the control group and the AMI model group, which underwent ligation of the left anterior descending coronary artery (LAD). The AMI animals were randomly divided into six experimental groups according to the duration of the myocardial infarction. All animals were subjected to ultrasound molecular imaging through injections with targeted microbubbles (T + T group) or nontargeted control microbubbles (T + C group). The values of the myocardial perfusion parameters (A, β, and A × β) were determined using Q-Lab (Philips ultrasound, version 9.0), and the expression level of SDF-1 was analyzed by real-time polymerase chain reaction (RT-PCR). Our results showed that the expression of SDF-1 gradually increased and peaked at 1 week after AMI. The trend is well reflected by ultrasound molecular imaging in the myocardial perfusion parameters. The A, β, and A × β values correlated with SDF-1 in the T + T group ( = 0.887, 0.892, and 0.942; < 0.05). Regression equations were established for the relationships of the A, β, and A × β values () with SDF-1 (): = 0.699 - 0.6048, = 0.4698 + 0.3282, and = 0.0945 + 0.6685, respectively ( = 0.772, 0.7957, and 0.8871; < 0.05). Our finding demonstrated that ultrasound molecular imaging could be used to evaluate the expression dynamics of SDF-1 after AMI.
急性心肌梗死（AMI）的干细胞治疗被证明是修复受损心肌的一种有前景的方法。干细胞移植的时间窗至关重要但难以确定，因为在心肌梗死后的不同时间进行移植会产生不同的治疗效果。基质细胞衍生因子-1（SDF-1）在移植干细胞的动员、归巢、增殖和分化中起关键作用。在此，我们通过使用超声分子成像靶向微泡，确定了SDF-1在急性心肌梗死猪模型中的动态表达。24只小型猪被随机分为对照组和急性心肌梗死模型组，后者接受左冠状动脉前降支（LAD）结扎。急性心肌梗死动物根据心肌梗死持续时间随机分为六个实验组。所有动物通过注射靶向微泡（T + T组）或非靶向对照微泡（T + C组）接受超声分子成像。使用Q-Lab（飞利浦超声，9.0版）测定心肌灌注参数（A、β和A×β）的值，并通过实时聚合酶链反应（RT-PCR）分析SDF-1的表达水平。我们的结果表明，SDF-1的表达在急性心肌梗死后逐渐增加，并在1周时达到峰值。这种趋势在超声分子成像的心肌灌注参数中得到了很好的体现。在T + T组中，A、β和A×β值与SDF-1相关（r = 0.887、0.892和0.942；P < 0.05）。建立了A、β和A×β值（y）与SDF-1（x）关系的回归方程：y = 0.699 - 0.6048x、y = 0.4698 + 0.3282x和y = 0.0945 + 0.6685x，分别为（r = 0.772、0.7957和0.8871；P < 0.05）。我们的发现表明，超声分子成像可用于评估急性心肌梗死后SDF-1的表达动态。
