Siekmann Wiebke, Tina Elisabet, Von Sydow Anita Koskela, Gupta Anil
Department of Anesthesiology and Intensive Care, University Hospital Örebro and School of Medical Sciences, Örebro University, SE-70185 Örebro, Sweden.
Department of Clinical Research Laboratory, Faculty of Medicine and Health, Örebro University, SE-70185 Örebro, Sweden.
Oncol Lett. 2019 Jul;18(1):395-401. doi: 10.3892/ol.2019.10332. Epub 2019 May 7.
Regional anesthesia may prolong survival following surgery for different types of cancers. The mechanisms behind this are unclear but direct effects on cancer cells by local anesthetics (LA) have been suggested. The aim of this study was to investigate if lidocaine or ropivacaine have a dose-dependent effect on the cell viability and proliferation of a primary and a secondary colon carcinoma cell line . The colon cancer cell lines SW480 derived from primary tumor and SW620 from a metastatic site in the same patient were exposed to increasing concentrations of lidocaine and ropivacaine (5-1,000 µM). Cell viability was measured using CellTiter-Blue and cell proliferation by PKH67 after exposure for up to 72 h. Cell viability was significantly reduced by ropivacaine at the highest concentration (1,000 µM) after 48 and 72 h in the cell line SW480 and at 72 h in SW620. Exposure to lidocaine did not show any significant reduction in cell viability. Notably, low concentrations of both lidocaine and ropivacaine significantly increased cell viability after 48 and 72 h in SW620. Cell proliferation was significantly reduced by 1,000 µM lidocaine in SW480 and by 1,000 µM ropivacaine in SW620. In summary, both lidocaine and ropivacaine showed an anti-proliferative effect in the colon cancer cell lines at high concentrations and after prolonged exposure to LA . Our findings also indicate that lower concentrations promote cell viability in the metastatic cell line.
区域麻醉可能会延长不同类型癌症手术后的生存期。其背后的机制尚不清楚，但有人提出局部麻醉药（LA）对癌细胞有直接作用。本研究的目的是调查利多卡因或罗哌卡因对原发性和继发性结肠癌细胞系的细胞活力和增殖是否有剂量依赖性影响。将源自原发性肿瘤的结肠癌细胞系SW480和来自同一患者转移部位的SW620暴露于浓度不断增加的利多卡因和罗哌卡因（5 - 1000 μM）中。暴露长达72小时后，使用CellTiter - Blue测量细胞活力，用PKH67测量细胞增殖。在细胞系SW480中，48小时和72小时后以及在SW620中72小时后，罗哌卡因在最高浓度（1000 μM）时细胞活力显著降低。暴露于利多卡因未显示细胞活力有任何显著降低。值得注意的是，在SW620中，48小时和72小时后，低浓度的利多卡因和罗哌卡因均显著提高了细胞活力。在SW480中，1000 μM利多卡因显著降低细胞增殖，在SW620中，1000 μM罗哌卡因显著降低细胞增殖。总之，高浓度且长时间暴露于局部麻醉药后，利多卡因和罗哌卡因在结肠癌细胞系中均显示出抗增殖作用。我们的研究结果还表明，较低浓度可促进转移性细胞系中的细胞活力。
