局部麻醉药罗哌卡因通过调节hsa_circ_0001320/miR-518a-5p轴抑制非小细胞肺癌进展。

Local anesthetic ropivacaine inhibits non-small cell lung cancer progression by modulating the hsa_circ_0001320/miR-518a-5p axis.

作者信息

Cheng LiQun, Shen RongRong, Ma Zhen, Guo Yuan, Peng Yi

机构信息

Department of Anesthesiology, Shanghai Children's Medical Center, Shanghai Jiaotong University School of Medicine, Shanghai City, 200127, China.

Department of Anesthesiology, Longhua Hospital Shanghai University of Traditional Chinese Medicine, No.725, Wanping South Road, Xuhui District, Shanghai City, 200032, China.

出版信息

Hereditas. 2025 Jul 25;162(1):142. doi: 10.1186/s41065-025-00514-4.

DOI:10.1186/s41065-025-00514-4

PMID:40713692

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12291326/

Abstract

OBJECTIVE

This study investigated the possible mechanisms by which ropivacaine influences the progression of non-small cell lung cancer (NSCLC).

METHODS

Plasmid vectors or oligonucleotides interfering with circ_0001320 or miR-518a-5p were transfected into ropivacaine-treated NSCLC cells, and circ_0001320 and miR-518a-5p levels were detected by RT-qPCR. Cell proliferation was assessed using CCK-8, apoptosis via flow cytometry, and cell migration and invasion through Transwell assays. Finally, the binding sites of circ_0001320 and miR-518a-5p were verified by the bioinformatics website CircInteractome and dual luciferase reporter gene assay.

RESULTS

Exposure to ropivacaine resulted in the suppression of NSCLC cell proliferation, migration, invasion, and angiogenesis, while inducing apoptosis. Ropivacaine elevated circ_0001320 expression. Circ_0001320 downregulation resulted in a reduced efficacy of ropivacaine in inhibiting NSCLC progression. Interestingly, circ_0001320 targeted miR-518a-5p and inhibited its expression. It was possible to limit the effects of downregulation of circ_0001320 on NSCLC progression by downregulating miR-518a-5p.

CONCLUSION

Ropivacaine inhibits NSCLC progression via the circ_0001320/miR-518a-5p axis.

摘要

目的

本研究探讨罗哌卡因影响非小细胞肺癌（NSCLC）进展的可能机制。

方法

将干扰circ_0001320或miR-518a-5p的质粒载体或寡核苷酸转染至经罗哌卡因处理的NSCLC细胞中，通过RT-qPCR检测circ_0001320和miR-518a-5p水平。使用CCK-8评估细胞增殖，通过流式细胞术检测细胞凋亡，并通过Transwell实验检测细胞迁移和侵袭。最后，通过生物信息学网站CircInteractome和双荧光素酶报告基因实验验证circ_0001320与miR-518a-5p的结合位点。

结果

罗哌卡因可抑制NSCLC细胞的增殖、迁移、侵袭和血管生成，同时诱导细胞凋亡。罗哌卡因可提高circ_0001320的表达。下调circ_0001320会导致罗哌卡因抑制NSCLC进展的效果降低。有趣的是，circ_0001320靶向miR-518a-5p并抑制其表达。下调miR-518a-5p可限制circ_0001320下调对NSCLC进展的影响。