da Rocha E M, Pereira L H, Do Rosario V E, Krettli A U
Department of Parasitology, University of Minas Gerais, Brazil.
Parassitologia. 1987 May-Dec;29(2-3):251-61.
After reviewing the use of non-human primates of the Old and New Worlds for human malaria research, we concluded that another experimental animal which is easily available to use and possible to rear indoors is needed. Thus, we studied the susceptibility of the marmoset Callithrix penicillata to Plasmodium falciparum erythrocytic infections. The marmosets received various P. falciparum human isolates, directly from a patient and from continuous cultures. The Palo Alto strain, which has been adapted to the night monkey Aotus trivirgatus and further maintained in the squirrel monkey Saimiri sciureus was also used. In a total of 20 marmosets we performed 31 inoculations, with 10(5) to 10(9) parasites, intraperitoneally, intracardiacly or intravenously. Blood samples from each animal were examined daily up to day 90 post-inoculation. None of the intact marmosets developed patent infections. Four out of 19 C. penicillata, previously splenectomized, showed circulating parasites for up to five days after intravenous inoculation with the Palo Alto strain, becoming negative thereafter. Neither the addition to the simian diet of p-aminobenzoic acid, essential for the parasite metabolism, nor drug-immunosuppression, improved the marmoset susceptibility to P. falciparum.
在回顾了新旧世界非人灵长类动物用于人类疟疾研究的情况后，我们得出结论，需要另一种易于获取且能够在室内饲养的实验动物。因此，我们研究了狨猴（Callithrix penicillata）对恶性疟原虫红细胞感染的易感性。这些狨猴接种了来自患者和连续培养物的各种恶性疟原虫人类分离株。还使用了已适应夜猴（Aotus trivirgatus）并在松鼠猴（Saimiri sciureus）中进一步传代保存的帕洛阿尔托株。我们总共对20只狨猴进行了31次接种，接种的疟原虫数量为10（5）至10（9）个，接种途径为腹腔内、心内或静脉内。在接种后长达90天的时间里，每天对每只动物的血液样本进行检查。所有完整的狨猴均未出现显性感染。19只先前已切除脾脏的狨猴中有4只在静脉接种帕洛阿尔托株后，循环中有疟原虫长达5天，此后转为阴性。无论是在猿类饮食中添加对寄生虫代谢至关重要的对氨基苯甲酸，还是进行药物免疫抑制，都没有提高狨猴对恶性疟原虫的易感性。
