Collins W E, Chin W, Warren M, Huong A Y, Jeffery G M, Skinner J C
J Parasitol. 1982 Aug;68(4):657-67.
Two strains of Plasmodium falciparum originating in Haiti were studied in the Aotus monkey. The Haitian I/CDC strain was first adapted to in vitro cultivation and subsequently inoculated into monkeys. The Haitian III/CDC strain was inoculated directly from a human patient into the Aotus monkey. The strains varied in their levels of pathogenicity to the animals. The Haitian I/CDC strain was highly virulent in six splenectomized animals; in one intact animal, the infection could be controlled but not eliminated with periodic doses of quinine and chloroquine. After subsequent splenectomy, the animal developed high parasitemias and died. No gametocytes developed in any of the Haitian I infections. The Haitian III strain was lethal to five of the 14 splenectomized monkeys inoculated, but some were able to control their infections without drug intervention. Gametocytes developed in all infections that persisted for an adequate length of time, and infections of mosquitoes were obtained both during the primary attack and the first recrudescence of the parasitemia. Of the mosquitoes tested, Anopheles freeborni was most susceptible to infection, followed by An. culicifacies, An. dirus, An. maculatus, and An. albimanus. The Haitian III strain was successfully transmitted to four other splenectomized Aotus monkeys via sporozoite inoculation using An. freeborni.
对源自海地的两株恶性疟原虫在夜猴身上进行了研究。海地I/疾病控制中心（CDC）株首先适应体外培养，随后接种到猴子体内。海地III/CDC株则直接从一名人类患者接种到夜猴体内。这两株疟原虫对动物的致病力水平有所不同。海地I/CDC株在六只脾切除的动物中具有高致病性；在一只未切除脾脏的动物中，感染可以通过定期给予奎宁和氯喹得到控制但无法消除。在随后进行脾切除后，该动物出现了高疟原虫血症并死亡。在任何海地I感染中均未出现配子体。海地III株对14只接种的脾切除猴子中的五只具有致死性，但有些猴子在没有药物干预的情况下能够控制感染。在所有持续足够长时间的感染中均出现了配子体，并且在初次发作和疟原虫血症的首次复发期间均获得了蚊子感染。在所测试的蚊子中，弗里博尼按蚊最易感染，其次是库氏按蚊、大劣按蚊、多斑按蚊和白跗按蚊。海地III株通过使用弗里博尼按蚊进行子孢子接种成功传播给了另外四只脾切除的夜猴。
