生长激素对生长激素释放激素基因表达的调节。
Growth hormone regulation of growth hormone-releasing hormone gene expression.
作者信息
de Gennaro Colonna V, Cattaneo E, Cocchi D, Müller E E, Maggi A
机构信息
Department of Pharmacology, School of Medicine, University of Milan, Italy.
出版信息
Peptides. 1988 Sep-Oct;9(5):985-8. doi: 10.1016/0196-9781(88)90077-0.
Slot-blot hybridization technique was used to evaluate growth hormone-releasing hormone (GHRH) mRNA levels in the hypothalamus of long-term (14 days) hypophysectomized (HPX) rats treated or not with 125 micrograms hGH/rat, twice daily IP, since the first day postsurgery. In addition, mRNA levels were determined in the hypothalamus of short-term (4 days) GH-treated (250 micrograms hGH/rat, twice daily IP) intact rats. GHRH mRNA levels were increased in HPX rats, and GH treatment partially counteracted this rise. Short-term administration of GH decreased GHRH mRNA levels in intact rats. These results, evaluated together with previous findings showing decreased hypothalamic GHRH-like immunoreactivity in both HPX rats and intact rats given GH (6, 7, 9), indicate that GH exerts a negative feedback action on the synthesis and release of GHRH.
采用狭缝杂交技术评估长期（14天）垂体切除（HPX）大鼠下丘脑内生长激素释放激素（GHRH）mRNA水平，这些大鼠自手术后第一天起，每天经腹腔注射两次，每次注射125微克hGH/大鼠，部分大鼠未接受该处理。此外，还测定了短期（4天）接受生长激素处理（250微克hGH/大鼠，每天经腹腔注射两次）的完整大鼠下丘脑内的mRNA水平。HPX大鼠的GHRH mRNA水平升高，生长激素处理可部分抵消这种升高。短期给予生长激素可降低完整大鼠的GHRH mRNA水平。这些结果与之前的研究结果（表明接受生长激素的HPX大鼠和完整大鼠下丘脑内GHRH样免疫反应性降低）一起评估，表明生长激素对GHRH的合成和释放具有负反馈作用。
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