Zimet Zlatko, Bilban Marjan, Osredkar Joško, Poljšak Borut, Fabjan Teja, Suhadolc Kristina
National Institute of Public Health, Ljubljana, Slovenia.
Department of Public Health, Faculty of Medicine, University of Ljubljana, Ljubljana, Slovenia.
Iran J Public Health. 2019 Jul;48(7):1284-1291.
We aimed to investigate the polluted working environment triggers oxidative stress and alter enzymatic antioxidant activity by a short-term interval.
The experimental study, performed in 2014, involved 94 workers from the Velenje Coalmine in Slovenia, arranged into three groups according to a number of consecutive working days in a mineshaft, supported by a control group. Levels of the antioxidant enzymes (GPx, CAT, SOD) together with TAC (the combined effect of all antioxidants) and 8-isoprostane (a biological marker of oxidative stress/damage) were measured in human plasma.
Workers occupationally exposed for three consecutive working days had significantly increased 8-isoprostane biomarker, a parameter of oxidative stress (<0.001). The antioxidant levels of TAC (<0.001), CAT (<0.001) and SOD (<0.001) were all significantly decreased compared to a control group.
Workers in polluted working environment had significantly increased oxidative stress and altered antioxidant activity already on a third consecutive working day.
我们旨在研究污染的工作环境是否会在短期内引发氧化应激并改变酶促抗氧化活性。
2014年进行的一项实验研究涉及斯洛文尼亚韦莱涅煤矿的94名工人，根据在矿井中连续工作的天数分为三组，并设有一个对照组。检测了人血浆中抗氧化酶（谷胱甘肽过氧化物酶、过氧化氢酶、超氧化物歧化酶）的水平以及总抗氧化能力（所有抗氧化剂的综合作用）和8-异前列腺素（氧化应激/损伤的生物标志物）。
连续三个工作日职业暴露的工人，其氧化应激参数8-异前列腺素生物标志物显著升高（<0.001）。与对照组相比，总抗氧化能力（<0.001）、过氧化氢酶（<0.001）和超氧化物歧化酶（<0.001）的抗氧化水平均显著降低。
在污染工作环境中的工人在连续第三个工作日时氧化应激就已显著增加，抗氧化活性也发生了改变。
