West China School of Public Health and West China Fourth Hospital, Sichuan University, Chengdu, China.
BioBank, The First Affiliated Hospital of Xi'an Jiaotong University, Shaanxi, China.
J Cell Mol Med. 2019 Nov;23(11):7342-7348. doi: 10.1111/jcmm.14593. Epub 2019 Sep 9.
Long non-coding RNA (lncRNA) is one of the important regulators of many malignancies. However, the biological function and clinical significance of a large number of lncRNAs in gastric cancer remain unclear. Therefore, we analysed the TCGA data to find that LINC01303 is significantly up-regulated in gastric cancer tissues. However, the biological function of LINC01303 in GC remains unknown. In our study, we found that the expression of LINC01303 was significantly higher in GC tissues than in adjacent tissues by real-time quantitative PCR. We can significantly inhibit the malignant proliferation, migration and invasion of GC cells by silencing LINC01303 expression. In addition, LINC01303 knockdown can also inhibit GC growth in vivo. After the bioinformatics analysis, we found that LINC01303 can be used as a miR-101-3p sponge to competitively adsorb miR-101-3p with EZH2. Therefore, our results indicate that LINC01303 promotes the expression of EZH2 by inhibiting miR-101-3p activity and promotes GC progression. In summary, in this study, we demonstrated for the first time that the LINC01303/miR-101-3p/EZH2 axis promotes GC progression.
长链非编码 RNA（lncRNA）是许多恶性肿瘤的重要调控因子之一。然而，大量 lncRNA 在胃癌中的生物学功能和临床意义仍不清楚。因此，我们分析了 TCGA 数据，发现 LINC01303 在胃癌组织中显著上调。然而，LINC01303 在 GC 中的生物学功能尚不清楚。在我们的研究中，通过实时定量 PCR 发现 LINC01303 在 GC 组织中的表达明显高于相邻组织。通过沉默 LINC01303 的表达，我们可以显著抑制 GC 细胞的恶性增殖、迁移和侵袭。此外，LINC01303 敲低也可以抑制 GC 在体内的生长。经过生物信息学分析，我们发现 LINC01303 可以作为 miR-101-3p 的海绵，与 EZH2 竞争结合 miR-101-3p。因此，我们的结果表明，LINC01303 通过抑制 miR-101-3p 的活性促进 EZH2 的表达，从而促进 GC 的进展。综上所述，在本研究中，我们首次证明了 LINC01303/miR-101-3p/EZH2 轴促进 GC 的进展。
