Zhuo Yue, Wang Xin, Wu Jingjie, Zhang Sujing, Deng Hongxiao, Liu Wei, Wu Hejian, Xiang Ping, Shen Min
Institute of Forensic Science, Guizhou Medical University, Guiyang, China.
Department of Forensic Toxicology, Shanghai Key Laboratory of Forensic Medicine, Shanghai Forensic Science Platform, Academy of Forensic Science, Shanghai, China.
J Forensic Sci. 2020 Mar;65(2):563-569. doi: 10.1111/1556-4029.14179. Epub 2019 Sep 9.
A method using liquid chromatography-tandem mass spectrometry (LC-MS/MS) to simultaneously quantify amphetamines, opiates, ketamine, cocaine, and metabolites in human hair is described. Hair samples (50 mg) were extracted with methanol utilizing cryogenic grinding. Calibration curves for all the analytes were established in the concentration range 0.05-10 ng/mg. The recoveries were above 72%, except for AMP at the limit of quantification (LOQ), which was 48%. The accuracies were within ±20% at the LOQ (0.05 ng/mg) and between -11% and 13.3% at 0.3 and 9.5 ng/mg, respectively. The intraday and interday precisions were within 19.6% and 19.8%, respectively. A proficiency test was applied to the validated method with z-scores within ±2, demonstrating the accuracy of the method for the determination of drugs of abuse in the hair of individuals suspected of abusing drugs. The hair concentration ranges, means, and medians are summarized for abused drugs in 158 authentic cases.
描述了一种使用液相色谱-串联质谱法（LC-MS/MS）同时定量人发中苯丙胺、阿片类药物、氯胺酮、可卡因及其代谢物的方法。利用低温研磨，用甲醇提取毛发样本（50毫克）。在0.05-10纳克/毫克的浓度范围内建立了所有分析物的校准曲线。除定量限（LOQ）处的苯丙胺回收率为48%外，其他回收率均高于72%。在定量限（0.05纳克/毫克）时，准确度在±20%以内，在0.3和9.5纳克/毫克时，准确度分别在-11%至13.3%之间。日内和日间精密度分别在19.6%和19.8%以内。对经过验证的方法进行了能力验证测试，z分数在±2以内，证明了该方法在测定疑似药物滥用者毛发中滥用药物时的准确性。总结了158例真实案例中滥用药物的毛发浓度范围、平均值和中位数。
