Tabibzadeh S S, Satyaswaroop P G
Department of Pathology, City Hospital Center, Elmhurst and Mount Sinai School of Medicine, City University of New York.
Am J Reprod Immunol Microbiol. 1988 Dec;18(4):124-30. doi: 10.1111/j.1600-0897.1988.tb00248.x.
We reported on the expression of HLA-DR molecules of the major histocompatibility complex in human endometrium. We now report on the expression of two other class II molecules, the HLA-DP and HLA-DQ determinants. These molecules were localized by monoclonal antibodies in 11 proliferative and 12 secretory endometria by avidin-biotin-complex (ABC) procedure. The expression of all three molecules was invariable and consistent throughout the entire menstrual cycle in endothelial and lymphoid cells. HLA-DR molecules were expressed in endometrial epithelium, particularly in the basalis in the mid to late proliferative phases of the cycle. In contrast, through the entire menstrual cycle, the expression of HLA-DP and HLA-DQ antigenic determinants was absent, and only occasionally a focal expression of these molecules was seen in endometrial epithelium. The in vitro induction of expression of the class II molecules in human endometrial epithelial cell cultures (HEE) by IFN-gamma was dose-dependent. After treatment with low doses of IFN-gamma, these cells primarily expressed HLA-DR molecules in vitro. The differential expression of the molecules of the major histocompatibility complex in human endometrial epithelium in vivo may be due to the differential sensitivity of th epithelium in response to cytokine(s).
我们报道了主要组织相容性复合体的HLA - DR分子在人子宫内膜中的表达情况。现在我们报道另外两种Ⅱ类分子，即HLA - DP和HLA - DQ决定簇的表达情况。通过抗生物素蛋白 - 生物素复合物（ABC）法，利用单克隆抗体对11例增殖期和12例分泌期子宫内膜中的这些分子进行定位。在内皮细胞和淋巴细胞中，这三种分子的表达在整个月经周期中是恒定且一致的。HLA - DR分子在内膜上皮中表达，尤其在周期增殖中期至晚期的基底层。相反，在整个月经周期中，HLA - DP和HLA - DQ抗原决定簇均无表达，仅偶尔在内膜上皮中可见这些分子的局灶性表达。γ干扰素在体外对人子宫内膜上皮细胞培养物（HEE）中Ⅱ类分子表达的诱导呈剂量依赖性。用低剂量γ干扰素处理后，这些细胞在体外主要表达HLA - DR分子。体内人子宫内膜上皮中主要组织相容性复合体分子的差异表达可能是由于上皮细胞对细胞因子反应的敏感性不同所致。
