Tabibzadeh S S, Gerber M A, Satyaswaroop P G
Am J Pathol. 1986 Oct;125(1):90-6.
Recent studies suggest that HLA-DR antigens may be inducible in some normal epithelial cells. Therefore, we studied the expression of these molecules in epithelial cell cultures derived from 9 proliferative and 7 secretory endometria with and without treatment by recombinant gamma-interferon (gamma-IFN). A panel of monoclonal antibodies to HLA-DR antigens, T cells, B cells, macrophages, endothelial cells, and cytokeratin was used to stain the cultured cells by the avidin-biotin complex method. The cultures consisted only of epithelial cells as demonstrated by expression of cytokeratin and by electron microscopy and did not contain any T or B cells, macrophages, or endothelial cells. The endometrial epithelial cells were HLA-DR-negative under routine culture conditions but expressed HLA-DR antigens after gamma-IFN treatment in a dose- and time-dependent fashion. The findings indicate that endometrial epithelium can actively synthesize HLA-DR antigens in vitro after induction by gamma-IFN.
近期研究表明，HLA - DR抗原可能在某些正常上皮细胞中被诱导产生。因此，我们研究了这些分子在来自9例增殖期和7例分泌期子宫内膜的上皮细胞培养物中的表达情况，这些培养物分别经过和未经过重组γ干扰素（γ - IFN）处理。使用一组针对HLA - DR抗原、T细胞、B细胞、巨噬细胞、内皮细胞和细胞角蛋白的单克隆抗体，通过抗生物素蛋白 - 生物素复合物法对培养细胞进行染色。如细胞角蛋白的表达及电子显微镜所示，培养物仅由上皮细胞组成，不包含任何T细胞、B细胞、巨噬细胞或内皮细胞。在常规培养条件下，子宫内膜上皮细胞HLA - DR呈阴性，但经γ - IFN处理后，以剂量和时间依赖的方式表达HLA - DR抗原。这些发现表明，子宫内膜上皮细胞在γ - IFN诱导后可在体外积极合成HLA - DR抗原。
