Biochemistry Department, Faculty of Science, University of Tabuk, Saudi Arabia; Bioinformatics Department, Genetic Engineering and Biotechnology Research Institute, University of Sadat City, Sadat City, Egypt.
Biochemistry Department, Faculty of Science, University of Tabuk, Saudi Arabia.
Int J Biol Macromol. 2019 Dec 1;141:511-516. doi: 10.1016/j.ijbiomac.2019.09.036. Epub 2019 Sep 6.
Fungal chitosan (ACT) extraction from Amylomyces rouxii, its transforming into nano-form, loading with fluconazole (Flu) and evaluation of synthesized nanoconjugates against drug-resistant (DR) Candida spp., were investigated. The produced ACT was characterized with 112.4 kDa molecular weight and 88.7% deacetylation degree. Synthesis of chitosan nanoparticles (NACT), and loading them with Flu were succeeded, using ionic gelation protocol, to generate stable Flu/NACT nanoconjugate' particles with mean size of 82 nm and zeta potential of +3.36 mV. The NACT entrapment efficiency was 78.7% and the drug loading capacity was 60.2%. Flu slowly released from NACT during the first 5 h, then release dramatically increased to the maximum (94.8%) after 12 h. The infra-red spectrum of Flu/NACT nanoconjugates confirmed the strong cross-linkage between their molecules. The antimycotic activity of NACT and Flu/NACT was proved against DR strains of C. albicans (2 strains), C. parapsilosis and C. glabrata, using qualitative and quantitative assays; Flu/NACT exhibited significant powerful activity, which was confirmed via observations with scanning microscopy. Finished cotton textiles with Flu/NACT had augmented potentiality for inhibiting challenged DR Candida spp., using in vitro assay. Accordingly, the synthesis and application of Flu/NACT nanoconjugates was astoundingly recommended for controlling DR Candida spp.
从粗糙脉孢菌中提取真菌壳聚糖（ACT），将其转化为纳米形式，负载氟康唑（Flu），并评价合成的纳米复合物对耐药（DR）念珠菌属的作用，进行了研究。所生产的 ACT 的分子量为 112.4 kDa，脱乙酰度为 88.7%。成功地使用离子凝胶化方案合成了壳聚糖纳米颗粒（NACT），并将其负载 Flu，以生成具有平均粒径为 82 nm 和 zeta 电位为+3.36 mV 的稳定 Flu/NACT 纳米复合物颗粒。NACT 的包封效率为 78.7%，载药量为 60.2%。Flu 在最初的 5 小时内从 NACT 缓慢释放，然后在 12 小时后急剧增加到最大值（94.8%）。Flu/NACT 纳米复合物的红外光谱证实了它们分子之间的强交联。通过定性和定量测定，证明了 NACT 和 Flu/NACT 对耐药性 C. albicans（2 株）、C. parapsilosis 和 C. glabrata 菌株的抗真菌活性；Flu/NACT 表现出显著的强大活性，通过扫描显微镜观察得到证实。使用体外试验，完成的带有 Flu/NACT 的棉纺织品对挑战耐药性念珠菌属的抑制具有增强的潜力。因此，强烈推荐合成和应用 Flu/NACT 纳米复合物来控制耐药性念珠菌属。
