阿魏酸通过抑制 NK-κB 和 MAPK 通路抑制 LPS 诱导的牛子宫内膜上皮细胞炎症反应。
Ferulic acid inhibits bovine endometrial epithelial cells against LPS-induced inflammation via suppressing NK-κB and MAPK pathway.
机构信息
College of Animal Science and Technology, Beijing University of Agriculture, Beijing 102206, China; College of Veterinary Medicine, China Agricultural University, Beijing 100094, China.
College of Animal Science and Technology, Beijing University of Agriculture, Beijing 102206, China.
出版信息
Res Vet Sci. 2019 Oct;126:164-169. doi: 10.1016/j.rvsc.2019.08.018. Epub 2019 Aug 12.
Endometritis is one of the most common reproductive diseases caused by bacterial infection in the cow. Ferulic acid is a major effective component extracted from Ligusticum wallichii. Ferulic acid displays pharmacological effects such as anti-inflammation and antioxidation. The aim of the present study was to investigate the protective effects of ferulic acid on inflammation induced by lipopolysaccharide (LPS) in bovine endometrial epithelial cells (BEECs). BEECs were pretreated with ferulic acid followed by LPS treatment. QRT-PCR analysis showed the mRNA expression of LPS-induced proinflammatory cytokines (IL1B, IL6, TNFA, and IL8) was decreased with ferulic acid pretreatment. Western blot analysis showed that ferulic acid inhibited the degradation of IκB and phosphorylation of NF-κB p65. Ferulic acid suppressed the phosphorylation of MAPKs, including p38 and JNK. All of these results indicated that ferulic acid may be considered as a potential anti-inflammatory drug for curing endometritis.
子宫内膜炎是奶牛由细菌感染引起的最常见的生殖系统疾病之一。阿魏酸是从当归中提取的主要有效成分。阿魏酸具有抗炎和抗氧化等药理作用。本研究旨在探讨阿魏酸对脂多糖（LPS）诱导的牛子宫内膜上皮细胞（BEEC）炎症的保护作用。用阿魏酸预处理 BEEC 后用 LPS 处理。实时定量 PCR 分析显示，阿魏酸预处理可降低 LPS 诱导的促炎细胞因子（IL1B、IL6、TNFA 和 IL8）的 mRNA 表达。Western blot 分析表明，阿魏酸抑制 IκB 的降解和 NF-κB p65 的磷酸化。阿魏酸抑制 MAPKs 的磷酸化，包括 p38 和 JNK。所有这些结果表明，阿魏酸可被视为治疗子宫内膜炎的一种有潜力的抗炎药物。
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