Bohácek J, Hosek B, Babusíková O, Hrivnáková A
Institute of Biophysics, Czechoslovak Academy of Sciences, Brno.
J Chromatogr. 1988 Dec 30;434(2):439-45.
Two methods for the determination of adenosine deaminase and purine nucleoside phosphorylase activities were compared. The high-performance liquid chromatographic (HPLC) technique used separation on a reversed-phase silica column and exhibited adequate sensitivity and a markedly higher rate of analysis compared with that of the paper radiochromatographic method. Correlation analysis of the results obtained by the two methods on a set of lymphoid cells from 25 patients with lympho-proliferative disorders confirmed the utility of the HPLC technique in clinical investigations.
比较了两种测定腺苷脱氨酶和嘌呤核苷磷酸化酶活性的方法。高效液相色谱（HPLC）技术采用反相硅胶柱进行分离，与纸放射色谱法相比，具有足够的灵敏度和明显更高的分析速率。对25例淋巴增殖性疾病患者的一组淋巴细胞采用这两种方法所得结果的相关性分析证实了HPLC技术在临床研究中的实用性。
