Toth K, Csik G, Averbeck D
Institute of Biophysics, Semmelweis Medical University, Budapest, Hungary.
J Photochem Photobiol B. 1988 Sep;2(2):209-20. doi: 10.1016/1011-1344(88)80004-6.
The monofunctional and bifunctional furocoumarin derivatives 8-methyl-3-carbethoxypsoralen (8Me3CPs) and 8-methoxypsoralen (8MOP) as well as their thiosubstituted derivatives (2-thio-8Me3CPs and 2-thio-8MOP) were compared in terms of their reactivities towards a ribonucleoprotein, the bacteriophage MS2. The order of their photoreactivities differed from that measured with nuclear DNA and mitochondrial DNA. Besides their widely investigated photoreactivity, their biological activity in the dark and after pre-irradiation was quantified. A parameter was defined which compares the number of molecules acting in the dark for 1 h with the number of absorbed photons which lead to the same degree of inactivation. The parameter for the furocoumarin derivatives examined, including 3-carbethoxypsoralen (3CPs) and 4'-aminomethyl-4,5',8-trimethylpsoralen (AMT), was in the following order: AMT greater than 3CPs greater than 8MOP greater than 8Me3CPs greater than 8Me3CPsS approximately 8MOPS. A similar parameter was also determined for the dark effect of pre-irradiated compounds.
对单功能和双功能呋喃香豆素衍生物8-甲基-3-乙氧羰基补骨脂素（8Me3CPs）、8-甲氧基补骨脂素（8MOP）及其硫代取代衍生物（2-硫代-8Me3CPs和2-硫代-8MOP）针对核糖核蛋白噬菌体MS2的反应活性进行了比较。它们的光反应活性顺序与用核DNA和线粒体DNA测得的顺序不同。除了对其广泛研究的光反应活性外，还对它们在黑暗中和预照射后的生物活性进行了量化。定义了一个参数，该参数将在黑暗中作用1小时的分子数量与导致相同失活程度的吸收光子数量进行比较。所检测的呋喃香豆素衍生物（包括3-乙氧羰基补骨脂素（3CPs）和4'-氨甲基-4,5',8-三甲基补骨脂素（AMT））的参数顺序如下：AMT＞3CPs＞8MOP＞8Me3CPs＞8Me3CPsS≈8MOPS。还针对预照射化合物的黑暗效应确定了类似的参数。
