Vedaldi D, Caffieri S, Miolo G, Dall'Acqua F, Arslan P
Department of Pharmaceutical Sciences, University of Padova.
Z Naturforsch C J Biosci. 1988 Nov-Dec;43(11-12):888-92. doi: 10.1515/znc-1988-11-1215.
It has been shown that various furocoumarins are able to cause dark hemolysis in red blood cells (RBC). However, this effect is evident only at relatively high furocoumarin concentrations (4.6 x 10(-4) M) - much higher than those used in photosensitization experiments or photochemotherapeutic treatments. Among the various furocoumarins examined in this study, only psoralen (Ps) and 3-carbethoxypsoralen (3-CPs) showed strong photohemolytic effects, while the other compounds revealed little or no activity. This fact indicates that Ps and 3-CPs are able to induce selective damage to the cell membrane of RBC. By pre-irradiating furocoumarin in ethanol or isotonic saline solutions and adding the irradiated solutions to a RBC suspension, hemolysis was observed in various compounds. The products of photolysis which form during pre-irradiation may be responsible, in terms of hemolysis, for toxic effects on RBC.
已表明，各种呋喃香豆素能够引起红细胞（RBC）的深色溶血。然而，这种效应仅在相对较高的呋喃香豆素浓度（4.6×10⁻⁴ M）下才明显——远高于光敏化实验或光化学治疗中使用的浓度。在本研究中检测的各种呋喃香豆素中，只有补骨脂素（Ps）和3 - 乙氧羰基补骨脂素（3 - CPs）表现出强烈的光溶血作用，而其他化合物几乎没有或没有活性。这一事实表明，Ps和3 - CPs能够诱导对RBC细胞膜的选择性损伤。通过在乙醇或等渗盐溶液中预照射呋喃香豆素，并将照射后的溶液加入RBC悬浮液中，在各种化合物中均观察到溶血现象。预照射过程中形成的光解产物，就溶血而言，可能对RBC产生毒性作用。
