Laboratory of Enzyme Engineering and Microbiology, University of Sfax, National Engineering School of Sfax, B.P. 1173, 3038 Sfax, Tunisia.
Laboratory of Enzyme Engineering and Microbiology, University of Sfax, National Engineering School of Sfax, B.P. 1173, 3038 Sfax, Tunisia; Higher Institute of Biotechnology of Monastir, University of Monastir, Monastir, Tunisia.
Mater Sci Eng C Mater Biol Appl. 2019 Nov;104:109867. doi: 10.1016/j.msec.2019.109867. Epub 2019 Jun 6.
The objective of this study was to prepare European eel oil (EO) microcapsules using European eel protein isolate (EPI) as a wall material and investigate its oxidative stability. The EPI emulsions were obtained at different EO: EPI ratios (1:1, 1:2 and 1:4, w/w) and using two emulsification procedures: Homogenization (H) and homogenization followed by ultrasonication (HU) treatments. The microcapsules prepared by combining the two emulsification processes (HU) and at core and wall ratio of was 1:4 presented the smallest particles size and the greatest encapsulation efficiency (68.50%) and oxidative stability. Scanning electron microscopy (SEM) images proved the spherical shape of all microcapsules without fissure on the surface. The capsules exhibited an interesting antioxidant activity depending on the EO:EPI ratio, especially for the metal chelating potential. Thus, the effect of ultrasonication process and the EPI concentration on the characteristic, the stability and the antioxidant activity of the encapsulated EO has been proved.
本研究旨在以欧洲鳗鱼蛋白分离物（EPI）为壁材制备欧洲鳗鱼油（EO）微胶囊，并研究其氧化稳定性。通过两种乳化工艺（均化处理（H）和均化处理后超声处理（HU）），在不同的 EO:EPI 比例（1:1、1:2 和 1:4，w/w）下获得 EPI 乳液。将两种乳化工艺（HU）结合使用，并将芯壁比设置为 1:4 制备的微胶囊，其粒径最小，包封效率（68.50%）和氧化稳定性最高。扫描电子显微镜（SEM）图像证实了所有微胶囊的球形形状，表面没有裂缝。胶囊表现出有趣的抗氧化活性，这取决于 EO:EPI 比例，特别是金属螯合潜力。因此，超声处理过程和 EPI 浓度对包封 EO 的特性、稳定性和抗氧化活性的影响得到了证实。
