Lou Zhewei, Gong Ting, Kang Jing, Xue Chao, Ulmschneider Christopher, Jiang Jack J
The Department of Otolaryngology-Head and Neck Surgery, Eye, Ear, Nose, and Throat Hospital of Fudan University, Shanghai, China.
Division of Otolaryngology-Head and Neck Surgery, The Department of Surgery, School of Medicine and Public Health, University of Wisconsin-Madison, Madison, Wisconsin.
Photobiomodul Photomed Laser Surg. 2019 Sep;37(9):532-538. doi: 10.1089/photob.2019.4641.
Photobiomodulation (PBM) is increasingly used in dermatology and dentistry due to its benefit of promoting wound healing and relieving pain; however, there is no corresponding research report on the application of PBM to vocal fold wound healing. To assess the potential wound-healing effects of PBM on the vocal folds via and experiments. In study, vocal fold fibroblasts (VFFs) were irradiated under a diode laser with wavelength of 635 nm at energy density of 8 J/cm. The Cell Counting Kit-8 (CCK-8) assay was used to study the viability of VFFs, and the gene expressions of , , , , and were investigated by real-time polymerase chain reaction (RT-PCR). In study, 15 rabbits were used. Lamina propria of the left vocal folds of 12 rabbits was unilaterally stripped, and 6 of them were treated with PBM. The remaining three rabbits served as normal controls. After 3 months, all animals were sacrificed to obtain histological results. We used laryngoscope to record images of the healing phase. Irradiation with energy density of 8 J/cm resulted in a 2.8% increase in cell proliferation ( < 0.05). However, the difference between the experimental and the control group became larger after 48 and 72 h of subsequent irradiation. RT-PCR results showed that the expression of , , and was higher, and the expression of and was lower. Histological examination showed that, compared with the injury group, hyaluronic acid (HA) increased significantly, collagen deposition decreased, and the configuration of collagen was more organized after PBM treatment. PBM can inhibit inflammatory reaction and promote the secretion of HA to decrease the deposition of collagen and regenerate vocal fold tissue without scar.
光生物调节（PBM）因其在促进伤口愈合和缓解疼痛方面的益处而越来越多地应用于皮肤科和牙科；然而，关于PBM在声带伤口愈合中的应用尚无相应的研究报告。通过[具体实验方式1]和[具体实验方式2]实验评估PBM对声带的潜在伤口愈合作用。在[实验一]研究中，将声带成纤维细胞（VFFs）置于波长为635nm、能量密度为8J/cm²的二极管激光下照射。采用细胞计数试剂盒-8（CCK-8）法研究VFFs的活力，并通过实时聚合酶链反应（RT-PCR）检测[相关基因1]、[相关基因2]、[相关基因3]、[相关基因4]和[相关基因5]的基因表达。在[实验二]研究中，使用了15只兔子。对12只兔子的左侧声带固有层进行单侧剥离，其中6只接受PBM治疗。其余3只兔子作为正常对照。3个月后，处死所有动物以获得组织学结果。我们使用喉镜记录愈合阶段的图像。能量密度为8J/cm²的照射导致细胞增殖增加2.8%（P<0.05）。然而，在随后照射48小时和72小时后，实验组与对照组之间的差异变得更大。RT-PCR结果显示，[相关基因1]、[相关基因2]和[相关基因3]的表达较高，而[相关基因4]和[相关基因5]的表达较低。组织学检查表明，与损伤组相比，PBM治疗后透明质酸（HA）显著增加，胶原沉积减少，胶原结构更有序。PBM可抑制炎症反应，促进HA分泌，减少胶原沉积，使声带组织再生且无瘢痕形成。
