Oropharynx and Larynx Service, Department of Otorhinolaryngology, Otorhinolaryngology Clinic, University of São Paulo School of Medicine, São Paulo, Brazil.
J Voice. 2012 Sep;26(5):667.e7-12. doi: 10.1016/j.jvoice.2011.08.007. Epub 2012 Jan 31.
OBJECTIVES/HYPOTHESIS: Hepatocyte growth factor (HGF) is a multifunctional polypeptide that plays various roles in embryogenesis and tissue regeneration and exhibits marked antifibrotic activity. The present study sought to assess the effects of HGF injection and reinjection coinciding with its peak of activity on collagen density, vessel density, inflammatory reaction in the lamina propria, and mean epithelial thickness in the injured rabbit vocal fold.
Prospective, controlled, experimental animal study.
Fourteen rabbits were subdivided into two groups and underwent injury of the vocal folds. Immediately after injury, animals in group 1 received HGF injections into the right vocal fold (RVF), whereas those in group 2 received bilateral HGF injections and a single reinjection into the RVF 10 days after the first, to coincide with the peak of HGF activity. The left vocal folds (LVFs) served as controls in both groups. Histological assessment of laryngeal specimens was performed at 30 and 40 days, respectively.
In both groups, collagen density was lower in the right (treated) vocal folds than in the left (control) folds (P=0.018). Vessel density was higher in the RVFs in group 2 (P=0.018). Differences were found in mean epithelial thickness and inflammatory reaction in the lamina propria but did not reach statistical significance.
In the scarred rabbit vocal fold, HGF injection is associated with decreased collagen density in the lamina propria, whereas reinjection after 10 days produces decreased collagen density and higher vessel density.
目的/假设：肝细胞生长因子（HGF）是一种多功能多肽，在胚胎发生和组织再生中发挥多种作用，并表现出显著的抗纤维化活性。本研究旨在评估 HGF 注射及其活性峰值时的再注射对受伤兔声带胶原密度、血管密度、固有层炎症反应和上皮平均厚度的影响。
前瞻性、对照、实验动物研究。
14 只兔子被分为两组，声带受伤。损伤后立即，第 1 组动物将 HGF 注射到右侧声带（RVF），而第 2 组动物接受双侧 HGF 注射，并在第 1 次注射后 10 天对 RVF 进行单次再注射，以与 HGF 活性峰值相吻合。两组的左侧声带（LVF）均作为对照。分别在 30 天和 40 天对喉标本进行组织学评估。
两组中，右侧（治疗）声带的胶原密度均低于左侧（对照）声带（P=0.018）。第 2 组的 RVF 血管密度较高（P=0.018）。在平均上皮厚度和固有层炎症反应方面存在差异，但未达到统计学意义。
在瘢痕形成的兔声带中，HGF 注射与固有层胶原密度降低有关，而 10 天后的再注射则导致胶原密度降低和血管密度升高。
