添加外源植酸酶的小剂量脂质基营养补充剂（SQ-LNS）对 Gambian 幼儿食用 SQ-LNS 小米粥后锌的分数和总吸收的影响：一项随机对照试验。

Effect of exogenous phytase added to small-quantity lipid-based nutrient supplements (SQ-LNS) on the fractional and total absorption of zinc from a millet-based porridge consumed with SQ-LNS in young Gambian children: a randomized controlled trial.

机构信息

Program in International and Community Nutrition, Department of Nutrition, University of California, Davis, Davis, CA, USA.

MRC Unit The Gambia at London School of Hygiene and Tropical Medicine, Banjul, The Gambia.

出版信息

Am J Clin Nutr. 2019 Dec 1;110(6):1465-1475. doi: 10.1093/ajcn/nqz205.

DOI:10.1093/ajcn/nqz205

PMID:31504101

Abstract

BACKGROUND

Dietary phytate inhibits zinc absorption from composite meals in adults.

OBJECTIVE

The objective of this study was to investigate the effect of adding exogenous phytase to a small-quantity lipid-based nutrient supplement (SQ-LNS) on zinc absorption among young children.

METHODS

In a double-blind randomized controlled trial, intraindividual differences in fractional and total absorption of zinc (FAZ and TAZ, respectively) from a millet-based porridge containing SQ-LNS with and without phytase were measured in 30 asymptomatic children 18-23 mo of age in the Kiang West district of The Gambia. Using a crossover design, children received for 1 d each porridge test meals with 20 g SQ-LNS containing 8 mg zinc and either 1) exogenous phytase or 2) no exogenous phytase. The test meals were provided on consecutive days in randomized order. FAZ was measured using a triple stable isotope tracer ratio technique with Zn-67 and Zn-70 as oral tracers and Zn-68 as the intravenous tracer.

RESULTS

Twenty-six participants completed the study. The prevalence of stunting and wasting were 20% and 13%, respectively; no children had low plasma zinc concentrations (<65 μg/dL). Total mean ± SD dietary zinc intake from the test meals was 7.3 ± 2.2 mg (phytate:zinc molar ratio = 3.1 ± 0.3, not accounting for phytase activity). Mean FAZ increased from 8.6% ± 1.3% to 16.0% ± 1.3% when exogenous phytase was added to the SQ-LNS product (P < 0.001). Mean TAZ from test meals containing SQ-LNS with phytase was more than double that from test meals containing SQ-LNS without phytase (1.1 ± 0.1 mg and 0.5 ± 0.1 mg, respectively; P < 0.001).

CONCLUSIONS

The addition of exogenous phytase to SQ-LNS increased both FAZ and TAZ. These results suggest that phytate reduction may be an important strategy to increase zinc absorption among young children. This trial was registered at clinicaltrials.gov as NCT02668133.

摘要

背景

膳食植酸会抑制成年人从复合膳食中吸收锌。

目的

本研究旨在探讨在小剂量脂质基营养素补充剂（SQ-LNS）中添加外源植酸酶对幼儿锌吸收的影响。

方法

在冈比亚基西西区进行的一项双盲随机对照试验中，30 名无症状 18-23 月龄儿童分别接受了含有 SQ-LNS 的小米粥（含 8mg 锌），其中含有和不含有外源植酸酶。采用交叉设计，每天食用一种粥测试餐，连续两天随机分配。使用三重稳定同位素示踪比技术，用 Zn-67 和 Zn-70 作为口服示踪剂，用 Zn-68 作为静脉内示踪剂，测量锌的分数吸收（FAZ）和总吸收（TAZ）。

结果

26 名参与者完成了研究。发育迟缓率和消瘦率分别为 20%和 13%，无儿童出现低血浆锌浓度（<65μg/dL）。测试餐的总平均锌摄入量为 7.3±2.2mg（植酸盐：锌摩尔比为 3.1±0.3，不考虑植酸酶活性）。当 SQ-LNS 产品中添加外源植酸酶时，FAZ 从 8.6%±1.3%增加到 16.0%±1.3%（P<0.001）。含有 SQ-LNS 与植酸酶的测试餐的平均 TAZ 是不含有 SQ-LNS 与植酸酶的测试餐的两倍多（分别为 1.1±0.1mg 和 0.5±0.1mg；P<0.001）。