Gandhi A P, Kjaergaard L
Biotechnol Bioeng. 1975 Aug;17(8):1109-18. doi: 10.1002/bit.260170803.
The influence of CO2 on the information of alpha-amylase by Bacillus subtilis NCIB 8646 growing in continuous and batch cultures was investigated. Different levels of CO2 examined in the batch cultures stimulated the formation of alpha-amylase, with the highest activity being obtained using 6% CO2 (v/v). The additions of CO2 inhibit the growth and division of vegetative cells of B. subtilis when CO2 is present in a concentration of more than 3% (v/v). In chemostat cultures, air containing 8% CO2 (v/v) increased the specific enzyme productivity almost three times over the control, without affecting the cell growth. An attempt is made to correlate the obtained results with the alesis.
研究了二氧化碳对枯草芽孢杆菌NCIB 8646在连续培养和分批培养中形成α-淀粉酶的影响。在分批培养中检测的不同二氧化碳水平刺激了α-淀粉酶的形成，使用6%（v/v）二氧化碳时获得了最高活性。当二氧化碳浓度超过3%（v/v）时，添加二氧化碳会抑制枯草芽孢杆菌营养细胞的生长和分裂。在恒化器培养中，含有8%（v/v）二氧化碳的空气使比酶生产率比对照提高了近三倍，而不影响细胞生长。试图将所得结果与损伤相关联。
