Trutko S M, Garagulia A D, Kiprianova E A, Akimenko V K
Mikrobiologiia. 1988 Nov-Dec;57(6):957-64.
The physiological role of pyocyanine for Pseudomonas aeruginosa was studied. Its synthesis was shown to commence at the retardation growth phase. Pyocyanine was accumulated only in the growth medium. The addition of 2,6-dichlorophenolindophenol accepting the reducing equivalents from coenzyme Q and transferring them to cytochrome c inhibited the pigment accumulation. This was indicative of the connection between pyocyanine synthesis and the level of the reducing equivalents in the cells. Pyocyanine did not accept the reducing equivalents from coenzyme Q in the respiratory chain of P. aeruginosa. Only reduced pyridine nucleotides served as substrates for pyocyanine in the reaction of autooxidation. The kinetic parameters of this reaction and the affinity of NADH dehydrogenase for the substrate were measured. The kinetic data were analysed to show that, under the physiological conditions, pyocyanine could not apparently compete with the respiratory chain for the reducing equivalents and hence directly regulate the level of NAD(P)H in P. aeruginosa cells. In order to keep the oxidising activity at a level necessary for the cells, the latter decreased the content of the reducing equivalents either by synthesizing pyocyanine or owing to the activity of cyanide-resistant oxidase. These processes of releasing the reducing equivalents are in a reciprocal relationship.
研究了绿脓菌素对铜绿假单胞菌的生理作用。结果表明，其合成始于生长迟滞期。绿脓菌素仅在生长培养基中积累。添加2,6 - 二氯酚靛酚，其从辅酶Q接受还原当量并将其转移至细胞色素c，抑制了色素积累。这表明绿脓菌素合成与细胞内还原当量水平之间存在联系。在铜绿假单胞菌的呼吸链中，绿脓菌素不接受来自辅酶Q的还原当量。在自氧化反应中，只有还原型吡啶核苷酸作为绿脓菌素的底物。测量了该反应的动力学参数以及NADH脱氢酶对底物的亲和力。对动力学数据的分析表明，在生理条件下，绿脓菌素显然不能与呼吸链竞争还原当量，因此不能直接调节铜绿假单胞菌细胞中NAD(P)H的水平。为了将氧化活性维持在细胞所需的水平，细胞通过合成绿脓菌素或由于抗氰化物氧化酶的活性来降低还原当量的含量。这些释放还原当量的过程呈相互关系。
