Nutman J, Chase P A, Dearborn D G, Berger M, Sorensen R U
Department of Pediatrics and Pathology, Case Western Reserve University School of Medicine, Cleveland, OH.
Isr J Med Sci. 1988 Apr-May;24(4-5):228-32.
Pseudomonas aeruginosa culture supernatants have been shown to inhibit lymphocytes proliferation as measured by [3H]TdR uptake. The phenazine pigment pyocyanine has been identified as one of the inhibitors present in those supernatants. To determine the mechanism of the inhibitory action of P. aeruginosa supernatants and pyocyanine, we studied their effect on the early stages of T-cell activation. Both P. aeruginosa supernatant and pyocyanine inhibited lymphocyte stimulation induced by the lectin concanavalin A. Analysis of Interleukin-2 receptor expression on the T-cell membrane showed that it was inhibited by both. This inhibition is dose dependent and not due to cellular toxicity. There was a parallel inhibition of growth in cell volume as well as [3H]TdR uptake. The results reported here suggest that P. aeruginosa culture supernatant and purified pyocyanine may interfere with cellular immune responses that may be necessary for eradication of chronic infection with P. aeruginosa in patients with cystic fibrosis.
铜绿假单胞菌培养上清液已被证明可抑制淋巴细胞增殖，这是通过[3H]TdR摄取来衡量的。吩嗪色素绿脓菌素已被确定为这些上清液中存在的抑制剂之一。为了确定铜绿假单胞菌上清液和绿脓菌素的抑制作用机制，我们研究了它们对T细胞活化早期阶段的影响。铜绿假单胞菌上清液和绿脓菌素均抑制了凝集素伴刀豆球蛋白A诱导的淋巴细胞刺激。对T细胞膜上白细胞介素-2受体表达的分析表明，两者均对其有抑制作用。这种抑制作用是剂量依赖性的，并非由细胞毒性引起。细胞体积的增长以及[3H]TdR摄取也受到了平行抑制。此处报道的结果表明，铜绿假单胞菌培养上清液和纯化的绿脓菌素可能会干扰细胞免疫反应，而这种反应对于根除囊性纤维化患者的铜绿假单胞菌慢性感染可能是必要的。
