Welsch U, Schumacher U
Department of Anatomy, University of Munich.
Acta Histochem Suppl. 1988;36:255-61.
Carbohydrate components were investigated in the bronchial glands and goblet cells of two species of seals with different diving habits (Weddell seal, Crabeater seal). Bouin-fixed tissue was incubated with various fluorescein-iso-thiocyanate-conjugated lectins (WGA, RCA I, PNA, PHA I, HPA, GSA I, DBA, Con A, LFA, SBA, UEA I), additionally PAS- and HID-reactions as well as Alcian Blue stains were applied. The two species differ in respect of their carbohydrate histochemistry, e.g., the PAS reaction gives only a faint stain at the apical membrane in the bronchial glands of the Weddell seal, whereas in the Crabeater seal several cells give a positive reaction in the entire cytoplasm; or RCA I has only few binding sites at the apical membranes of the bronchial glandular cells of the Weddell seal whereas many cells of Crabeater bronchial glands react strongly positive. It is concluded that the secretory products of the bronchial glands of the long- and deep-diving Weddell seal are relatively poor in mucins, which is in contrast to other mammals studied so far.
对两种具有不同潜水习性的海豹（威德尔海豹、南极毛皮海狮）的支气管腺和杯状细胞中的碳水化合物成分进行了研究。用各种异硫氰酸荧光素偶联凝集素（WGA、RCA I、PNA、PHA I、HPA、GSA I、DBA、Con A、LFA、SBA、UEA I）孵育经布因固定的组织，另外还进行了PAS反应、高铁二胺反应以及阿尔辛蓝染色。这两个物种在碳水化合物组织化学方面存在差异，例如，PAS反应在威德尔海豹支气管腺的顶端膜处仅产生微弱染色，而在南极毛皮海狮中，几个细胞在整个细胞质中呈阳性反应；或者RCA I在威德尔海豹支气管腺细胞的顶端膜上只有很少的结合位点，而南极毛皮海狮支气管腺的许多细胞反应呈强阳性。得出的结论是，长时间深潜的威德尔海豹支气管腺的分泌产物中粘蛋白相对较少，这与迄今为止研究的其他哺乳动物形成对比。
