Pastor L M, Ferran A, Calvo A, Sprekelsen C, Horn R, Marin J A
Department of Cell Biology, Medical School, University of Murcia, Spain.
Anat Rec. 1994 Aug;239(4):453-67. doi: 10.1002/ar.1092390411.
The respiratory submucosal glands are a major source of secretions in the airway. Human submucosal laryngeal glands have been scarcely studied, with no works existing about their ultrastructure and histochemistry.
Samples of epiglottis, ventricle, false vocal folds and true vocal folds were fixed in 10% buffered formalin for histochemical study with conventional and carbohydrate lectin histochemistry. Other samples were fixed in 2.5% glutaraldehyde and conventionally processed for transmission electron microscopy.
The human submucosal laryngeal glands are composed of serious tubules; mucous tubules; collector duct; and final portion of this duct. The serous cells showed sialosulphomucins and affinity for WGA and Con-A lectins. With a previous treatment with neuraminidase, they also labelled with PNA. The mucous cells contained sialosulphomucins and showed affinity for WGA and DBA lectins in the samples proceeding from blood group A, and for WGA, UEA-I and LTA with those from blood group O. Ultrastructurally, the serous cells presented a wide variety of granules, cells in which seromucous granules predominated. The mucous cells presented larger-sized granules which were very electron-lucent. The collector duct was composed of mitochondria-rich cells and basal cells. A cell which we have termed "intermediate" was identified in the transition zone between the mucous tubules and the collector duct, and in the final portion of the collector duct. It had morphological characteristics as if it were a transition between a goblet cell and collector duct cell. Some nerve endings with cholinergic and peptidergic vesicles were found among the myoepithelial cells.
These glands presented some histological differences from the bronchial glands, the mucous secretion was related to the blood group antigens, and the serous cells showed a wide variability in their secretory granules, many of them being of a seromucous type.
呼吸道黏膜下腺是气道分泌物的主要来源。人类喉黏膜下腺的研究极少，尚无关于其超微结构和组织化学的研究报道。
会厌、室带、假声带和真声带样本用10%中性福尔马林固定，进行常规组织化学和碳水化合物凝集素组织化学研究。其他样本用2.5%戊二醛固定，常规处理后用于透射电子显微镜观察。
人类喉黏膜下腺由浆液性腺管、黏液性腺管、集合管及该导管的终末部分组成。浆液性腺细胞显示含有涎酸硫酸黏蛋白，对麦胚凝集素（WGA）和刀豆球蛋白A（Con - A）凝集素有亲和力。经神经氨酸酶预处理后，它们也与花生凝集素（PNA）结合。黏液性腺细胞含有涎酸硫酸黏蛋白，来自A型血样本的黏液性腺细胞对WGA和双花扁豆凝集素（DBA）凝集素有亲和力，来自O型血样本的黏液性腺细胞对WGA、荆豆凝集素I（UEA - I）和扁豆凝集素（LTA）有亲和力。超微结构上，浆液性腺细胞呈现多种颗粒，以浆液黏液性颗粒为主的细胞。黏液性腺细胞呈现较大的颗粒，电子密度很低。集合管由富含线粒体的细胞和基底细胞组成。在黏液性腺管和集合管的过渡区以及集合管的终末部分发现一种我们称为“中间型”的细胞。它具有形态学特征，似乎是杯状细胞和集合管细胞之间的过渡类型。在肌上皮细胞之间发现了一些含有胆碱能和肽能小泡的神经末梢。
这些腺体与支气管腺体存在一些组织学差异，黏液分泌与血型抗原有关，浆液性腺细胞的分泌颗粒表现出很大的变异性，其中许多是浆液黏液性类型。
