Boujaafar N, Jeddi M, Freney J
Laboratoire de Microbiologie, C.H.U.F. Hached, Sousse, Tunisie.
Arch Inst Pasteur Tunis. 1988 Jul-Oct;65(3-4):271-8.
A rapid and simple method for preparation of chromosomal DNA from Gram-positive bacteria is reported. Susceptibility to lysis with Sodium Dodecyl Sulfate (SDS) increases when undergoing treatment with acetone before being digested by bacteriolytic enzymes. Rapid lysis of Staphylococcus and Listeria cells is obtained through a respective treatment by lysozyme with lysostaphine and by lysozyme with achromopeptidase, adding to that the effect of SDS in Tris-Hcl buffer. This procedure of preparing chromosomal DNA provides 1 to 4 mg of DNA out of 1 g of bacterial cells in a day.
报道了一种从革兰氏阳性菌中制备染色体DNA的快速简便方法。在用溶菌酶消化之前，丙酮处理可增加革兰氏阳性菌对十二烷基硫酸钠（SDS）裂解的敏感性。通过溶菌酶分别与溶葡萄球菌素以及溶菌酶与无色肽酶处理葡萄球菌和李斯特菌细胞，可实现快速裂解，此外SDS在Tris-Hcl缓冲液中也有作用。这种制备染色体DNA的方法一天内可从1克细菌细胞中获得1至4毫克DNA。
