Barsotti O, Renaud F, Freney J, Benay G, Decoret D, Dumont J
Laboratoire de Microbiologie-Immunologie, Faculté d'Odontologie, Lyon, France.
Ann Inst Pasteur Microbiol. 1987 Sep-Oct;138(5):529-36. doi: 10.1016/0769-2609(87)90038-x.
DNA could not be quickly extracted from members of the genus Actinomyces by the usual methods of lysis. Treatment of 7 different actinomyces cells with lysozyme and achromopeptidase, both 5 mg/g wet cells, for 2 h, followed by SDS (0.2%), proteinase K (5 mg/g wet cells) and EDTA (lmM) for 1 h, lysed the cells. The yield obtained in one day was 337 micrograms per 200 mg of bacterial cells. The treatment was also found to work effectively on strains belonging to Veillonella, Staphylococcus, Fusobacterium and Bifidobacterium genera.
采用常规的裂解方法无法从放线菌属成员中快速提取DNA。用溶菌酶和无色肽酶（均为5mg/g湿细胞）处理7种不同的放线菌细胞2小时，随后用0.2%的十二烷基硫酸钠（SDS）、5mg/g湿细胞的蛋白酶K和1mM的乙二胺四乙酸（EDTA）处理1小时，可使细胞裂解。一天内每200mg细菌细胞的产量为337微克。还发现该处理方法对韦荣球菌属、葡萄球菌属、梭杆菌属和双歧杆菌属的菌株也有效。
