Department of Hormonal Action Mechanisms, Institute of Animal Reproduction and Food Research of the Polish Academy of Sciences, Olsztyn, Poland.
Sci Rep. 2019 Sep 10;9(1):12968. doi: 10.1038/s41598-019-49502-5.
Previous studies highlighted chemokines as potential factors regulating changes in the endometrium during early pregnancy. The current study aimed to screen the effects of a broad range of chemokines and indicate those that are involved in porcine luminal epithelial (LE) cell remodelling. Messenger RNA expression of chemokines (CCL2, CCL4, CCL5, CCL8, CXCL2, CXCL8, CXCL10 and CXCL12) and both the mRNA and protein expression of their receptors (CCR1, CCR2, CCR3, CCR5, CXCR2, CXCR3, CXCR4) were detected in LE cells. Exogenous CCL8 enhanced the proliferative and migration potential of LE cells and their motility in the environment with its stable concentration. The adhesive properties of LE cells were negatively affected by CCL8. However, CXCL12 positively affected the proliferation, motility and adhesion of LE cells as well as caused a decrease in MUC1 mRNA expression. To conclude, our studies determined that exogenous chemokines affected critical endometrial epithelial cell functions in the context of embryo implantation. We suggest that of all the examined factors, chemokine CCL8 participates in the establishment of a proper environment for embryo implantation, whereas CXCL12, apart from participation in endometrial receptivity, promotes embryo attachment.
先前的研究强调趋化因子是调节妊娠早期子宫内膜变化的潜在因素。本研究旨在筛选广泛的趋化因子的作用，并指出那些参与猪腔上皮（LE）细胞重塑的趋化因子。检测了趋化因子（CCL2、CCL4、CCL5、CCL8、CXCL2、CXCL8、CXCL10 和 CXCL12）及其受体（CCR1、CCR2、CCR3、CCR5、CXCR2、CXCR3、CXCR4）的信使 RNA 表达及其 mRNA 和蛋白表达。外源性 CCL8 增强了 LE 细胞的增殖和迁移潜力及其在其稳定浓度环境中的迁移能力。LE 细胞的黏附特性受到 CCL8 的负面影响。然而，CXCL12 可正向影响 LE 细胞的增殖、迁移和黏附，还可导致 MUC1 mRNA 表达降低。综上所述，我们的研究确定了外源性趋化因子在胚胎着床背景下影响关键子宫内膜上皮细胞功能。我们认为，在所有检查的因素中，趋化因子 CCL8 参与为胚胎着床建立适当的环境，而 CXCL12 除了参与子宫内膜容受性外，还促进胚胎附着。
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