Mei Xiuli, Xu Ling, Ren Yan, Yu Minjie, Kuang Liangde, Li Congyan, Zhang Yan, Lu Chuanzhi, Wang Zhicheng, Guo Zhiqiang, Xie Xiaohong, Huang Dengping, Zhang Ming
Animal Breeding and Genetics Key Laboratory of Sichuan Province, Sichuan Animal Science Academy, Chengdu, China.
College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, China.
Front Vet Sci. 2022 Mar 10;9:838802. doi: 10.3389/fvets.2022.838802. eCollection 2022.
The chorion from the placenta is directly attached to the endometrium (CA) after embryo implantation while some parts of the endometrium are not chorion-attached (NCA). The differences in gene expression between the CA and NCA endometrium mid-gestation are unknown. Our objective was to compare the gene expression profiles of the CA and NCA endometrium of rabbit, to identify the differentially expressed genes (DEGs), and correlate the differences with the physiological state of the endometrium at mid-gestation of rabbit.
We used transcriptome sequencing to reveal the differences in gene expression between CA and NCA endometrium ( = 3), and then determined the concentration of inflammatory cytokines in CA and NCA tissue and serum by ELISA.
Six Hundred and Forty-Six DEGs were identified between the CA and NCA endometrium [ < 0.05, |log2 (fold change) |≥ 2], The expression levels of 590 DEGs were higher in the NCA endometrium than in the CA endometrium, while the expression level of only 56 DEGs were higher in CA than in NCA. The DEGs were enriched in gene ontology (GO) terms and pathways related to immune regulation and cellular adhesions. Six hub-genes related to inflammatory mediator regulation of transient receptor potential (TRP) channels and chemokine signaling pathways had a lower expression level in the CA endometrium compared to the NCA endometrium, and the expression levels of genes related to focal adhesion and extracellular matrix (ECM)-receptors were significantly higher in NCA endometrium than in CA endometrium. The level of pro-inflammatory cytokines accumulated in the CA endometrium, and high abundance of integrin-β and THBS1 were localized in the luminal epithelium of the NCA endometrium, but not in the CA endometrium.
Our study reveals differences in gene expression between the CA and NCA endometrium at mid-gestation of rabbit, and suggests implications for endometrial physiological function. The CA endometrium showed relative low-level gene expression compared to the NCA endometrium, while the NCA endometrium performed physiological functions related to focal adhesion and ECM-receptor interaction.
胚胎着床后，胎盘的绒毛膜直接附着于子宫内膜（CA），而子宫内膜的某些部分未附着绒毛膜（NCA）。妊娠中期CA和NCA子宫内膜之间基因表达的差异尚不清楚。我们的目的是比较兔CA和NCA子宫内膜的基因表达谱，鉴定差异表达基因（DEG），并将这些差异与兔妊娠中期子宫内膜的生理状态相关联。
我们使用转录组测序来揭示CA和NCA子宫内膜（n = 3）之间基因表达的差异，然后通过ELISA测定CA和NCA组织及血清中炎性细胞因子的浓度。
在CA和NCA子宫内膜之间鉴定出646个DEG（P < 0.05，|log2（倍数变化）|≥ 2），590个DEG在NCA子宫内膜中的表达水平高于CA子宫内膜，而只有56个DEG在CA中的表达水平高于NCA。这些DEG在与免疫调节和细胞黏附相关的基因本体（GO）术语和途径中富集。与瞬时受体电位（TRP）通道的炎性介质调节和趋化因子信号通路相关的6个枢纽基因在CA子宫内膜中的表达水平低于NCA子宫内膜，与粘着斑和细胞外基质（ECM）-受体相关的基因在NCA子宫内膜中的表达水平显著高于CA子宫内膜。促炎细胞因子在CA子宫内膜中积累，整合素-β和血小板反应蛋白-1（THBS1）的高丰度定位于NCA子宫内膜的腔上皮，而不在CA子宫内膜中。
我们的研究揭示了兔妊娠中期CA和NCA子宫内膜之间的基因表达差异，并提示了对子宫内膜生理功能的影响。与NCA子宫内膜相比，CA子宫内膜显示出相对低水平的基因表达，而NCA子宫内膜执行与粘着斑和ECM-受体相互作用相关的生理功能。
