Khettab N, Amory M C, Briand G, Bousquet B, Combre A, Forlot P, Barey M
Laboratoire de Biochimie et de Physiologie, Faculté de Pharmacie, Nantes, France.
Biochimie. 1988 Dec;70(12):1709-13. doi: 10.1016/0300-9084(88)90028-4.
The purpose of this study was to confirm the photoprotective effect on skin of vitamins A and E, due to inhibition of polyamine synthesis and production of free radicals. These variables were measured in the lumbar epidermis of the female hairless mouse subjected to UVA + B irradiation. Polyamines were assayed in epidermal homogenate by HPLC, and production of oxygenated free radicals was determined by spectrofluorometric assay of malonyl dialdehyde. It was determined that butyl-hydroxy-toluene and vitamin E inhibited production of free radicals (56% and 60%, respectively) and caused a significant reduction in polyamine biosynthesis (P less than 0.01), whereas the inhibitory effect of malonyl dialdehyde induced by vitamin A (30%) had no associated effect on polyamine metabolism.
本研究的目的是通过抑制多胺合成和自由基产生来证实维生素A和E对皮肤的光保护作用。在接受UVA + B照射的雌性无毛小鼠的腰部表皮中测量这些变量。通过高效液相色谱法测定表皮匀浆中的多胺，并通过丙二醛的荧光分光光度法测定氧化自由基的产生。结果表明，丁基羟基甲苯和维生素E抑制自由基产生（分别为56%和60%），并导致多胺生物合成显著减少（P小于0.01），而维生素A诱导的丙二醛抑制作用（30%）对多胺代谢没有相关影响。
