Zhai Zhongying, Du Jiao, Chen Lijie, Hamid Muhammad Rizwan, Du Xiaohua, Kong Xiaoting, Cheng Jue, Tang Wen, Zhang Deyong, Su Pin, Liu Yong
Longping Branch, Graduate School of Hunan University, Changsha, 410125, China.
Hunan Plant Protection Institute, Hunan Academy of Agricultural Sciences, Changsha, 410125, China.
AMB Express. 2019 Sep 10;9(1):141. doi: 10.1186/s13568-019-0866-6.
Development of a genetic tool for visualization of photosynthetic bacteria (PSB) is essential for understanding microbial function during their interaction with plant and microflora. In this study, Rhodopseudomonas palustris GJ-22-gfp harboring the vector pBBR1-pckA-gfp was constructed using an electroporation transformation method and was used for dynamic tracing of bacteria in plants. The results showed that strain GJ-22-gfp was stable and did not affect the biocontrol function, and the Confocal Laser Scanning Microscopy (CLSM) results indicated it could successfully colonised on the surface of leaf and root of tobacco and rice. In tobacco leaves, cells formed aggregates on the mesophyll epidermal cells. While in rice, no aggregate was found. Instead, the fluorescent cells colonise the longitudinal intercellular spaces between epidermal cells. In addition, the results of strain GJ-22 on the growth promotion and disease resistance of tobacco and rice indicated that the different colonization patterns might be related to the bacteria could induce systemic resistance in tobacco.
开发一种用于可视化光合细菌（PSB）的遗传工具对于理解微生物在与植物和微生物群落相互作用过程中的功能至关重要。在本研究中，使用电穿孔转化方法构建了携带载体pBBR1-pckA-gfp的沼泽红假单胞菌GJ-22-gfp，并用于动态追踪植物中的细菌。结果表明，菌株GJ-22-gfp稳定且不影响生防功能，共聚焦激光扫描显微镜（CLSM）结果表明它能成功定殖在烟草和水稻的叶和根表面。在烟草叶片中，细胞在叶肉表皮细胞上形成聚集体。而在水稻中，未发现聚集体。相反，荧光细胞定殖在表皮细胞之间的纵向细胞间隙中。此外，菌株GJ-22对烟草和水稻生长促进和抗病性的结果表明，不同的定殖模式可能与该细菌能诱导烟草产生系统抗性有关。
