重构膜泡的同种细胞溶解作用。
Allogeneic cytolysis of reconstituted membrane vesicles.
作者信息
Hollander N, Mehdi S Q, Weissman I L, McConnell H M, Kriss J P
出版信息
Proc Natl Acad Sci U S A. 1979 Aug;76(8):4042-5. doi: 10.1073/pnas.76.8.4042.
Abstract
The successful use of lipid bilayer model membranes as targets for cytotoxic lymphocytes is described. Lipid vesicles were made from a mixture of dipalmitoyl lecithin, dimyristoyl lecithin, and cholesterol. Membrane proteins of LSTRA or EL4 tumor cells (as source of H-2 antigens), human eye muscle membrane proteins (as supporting proteins), and 51Cr marker were inserted into the lipid vesicles. Incubation of the reconstituted vesicles with lymphocytes sensitized in mixed lymphocyte cultures against allogeneic cells resulted in the specific release of intravesicular 51Cr. Vesicle damage was mediated by thymus-derived lymphocytes. H-2 antigens could be incorporated into vesicles without eye muscle proteins. However, immune damage of the vesicles could not be demonstrated when vesicles inserted with H-2 antigens in the absence of eye muscle proteins were used as targets.
摘要
本文描述了脂质双层模型膜作为细胞毒性淋巴细胞靶标的成功应用。脂质囊泡由二棕榈酰卵磷脂、二肉豆蔻酰卵磷脂和胆固醇的混合物制成。将LSTRA或EL4肿瘤细胞的膜蛋白（作为H-2抗原的来源）、人眼肌膜蛋白（作为支持蛋白）和51Cr标记物插入脂质囊泡中。将重组后的囊泡与在混合淋巴细胞培养中针对同种异体细胞致敏的淋巴细胞一起孵育，导致囊泡内51Cr的特异性释放。囊泡损伤由胸腺来源的淋巴细胞介导。H-2抗原可以在没有眼肌蛋白的情况下掺入囊泡中。然而，当使用在没有眼肌蛋白的情况下插入H-2抗原的囊泡作为靶标时，未观察到囊泡的免疫损伤。
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