Lin Xiaoyuan, Pavani Krishna Chaitanya, Smits Katrien, Deforce Dieter, Heindryckx Björn, Van Soom Ann, Peelman Luc
Department of Nutrition, Genetics and Ethology, Faculty of Veterinary Medicine, Ghent University, Ghent, Belgium.
Reproduction, Obstetrics and Herd Health, Ghent University, Ghent, Belgium.
Front Genet. 2019 Aug 22;10:757. doi: 10.3389/fgene.2019.00757. eCollection 2019.
In a previous study, we found miR-10b to be more abundant in a conditioned culture medium of degenerate embryos compared to that of blastocysts. Here, we show that miR-10b mimics added to the culture medium can be taken up by embryos. This uptake results in an increase in embryonic cell apoptosis and aberrant expression of DNA methyltransferases (). Using several algorithms, Homeobox A1 () was identified as one of the potential miR-10b target genes and dual-luciferase assay confirmed as a direct target of miR-10b. Microinjection of si- into embryos also resulted in an increase in embryonic cell apoptosis and downregulation of . Cell progression analysis using Madin-Darby bovine kidney cells (MDBKs) showed that miR-10b overexpression and knockdown results in suppressed cell cycle progression and decreased cell viability. Overall, this work demonstrates that miR-10b negatively influences embryo quality and might do this through targeting and/or influencing DNA methylation.
在之前的一项研究中，我们发现与囊胚相比，退化胚胎的条件培养基中miR-10b更为丰富。在此，我们表明添加到培养基中的miR-10b模拟物可被胚胎摄取。这种摄取导致胚胎细胞凋亡增加以及DNA甲基转移酶的异常表达。使用多种算法，同源盒A1（）被鉴定为潜在的miR-10b靶基因之一，双荧光素酶测定证实为miR-10b的直接靶标。向胚胎中显微注射si-也导致胚胎细胞凋亡增加以及的下调。使用马-达二氏牛肾细胞（MDBK）进行的细胞进程分析表明，miR-10b过表达和敲低导致细胞周期进程受抑制以及细胞活力下降。总体而言，这项工作表明miR-10b对胚胎质量有负面影响，并且可能通过靶向和/或影响DNA甲基化来实现。
