Yousefi Behpour, Rahbar Elnaz
Abnormal Uterine Bleeding Research Center, Semnan University of Medical Sciences, Semnan, Iran.
Department of Anatomy, Medicine Faculty, Semnan University of Medical Science, Semnan, Iran.
Int J Reprod Biomed. 2019 Jul 29;17(6):385-394. doi: 10.18502/ijrm.v17i6.4809. eCollection 2019 Jun.
Clomiphene citrate (CC) is one of the widely used drugs as an ovulation stimulant, but its adverse effects on the endometrium results in lowering down the pregnancy rate. Endometrium CD98 is also important in the process of implantation.
To evaluate the immunohistochemistry expression levels of endometrial CD98 following injection of CC with and without Human chorionic gonadotropin (HCG) in ovariectomized and non-ovariectomized rats.
Seventy two (12-14 wk old) female Wistar rats were randomly divided into two groups (n = 36): (a) ovariectomized and (b) non-ovariectomized. Each group was further divided into six subgroups (n = 6/each): (1) CC 10 mg/kg, (2) CC 20 mg/kg, (3) HCG, (4) CC 10 mg/kg with HCG, (5) CC 20 mg/kg with HCG, and (6) control. The experimental subgroups received a single dose of CC (daily, five days) and HCG (after the last injection of CC) alone or in combination. Immunohistochemistry staining was performed on paraffin-embedded endometrial tissues to evaluate the expression levels of CD98.
Animals undergoing ovariectomy presented a significantly lower expression level of endometrial CD98 (p 0.001) when compared with non-ovariectomized in the same condition that groups were subdivided. There was also a dose-dependent reduction (p 0.001) in the expression of CD98 in non-ovariectomized subgroups when compared with control group. In addition, injection of HCG following treatment with CC improved its expression.
It was concluded that CC impairs CD98 expression in endometrium and this impairment is intensified the removal of the ovary. Also, an injection of HCG following treatment with CC can slightly improve the expression of CD98.
枸橼酸氯米芬（CC）是一种广泛用于刺激排卵的药物，但其对子宫内膜的不良影响会导致妊娠率降低。子宫内膜CD98在着床过程中也很重要。
评估在去卵巢和未去卵巢大鼠中注射CC联合或不联合人绒毛膜促性腺激素（HCG）后子宫内膜CD98的免疫组化表达水平。
72只（12 - 14周龄）雌性Wistar大鼠随机分为两组（n = 36）：（a）去卵巢组和（b）未去卵巢组。每组再进一步分为六个亚组（n = 6/每组）：（1）CC 10 mg/kg，（2）CC 20 mg/kg，（3）HCG，（4）CC 10 mg/kg联合HCG，（5）CC 20 mg/kg联合HCG，以及（6）对照组。实验亚组单独或联合接受单剂量的CC（每日，共五天）和HCG（在最后一次注射CC后）。对石蜡包埋的子宫内膜组织进行免疫组化染色，以评估CD98的表达水平。
在相同分组条件下比较，去卵巢动物的子宫内膜CD98表达水平显著低于未去卵巢动物（p < 0.001）。与对照组相比，未去卵巢亚组中CD98的表达也呈剂量依赖性降低（p < 0.001）。此外，CC治疗后注射HCG可改善其表达。
得出结论，CC会损害子宫内膜中CD98的表达，且这种损害在卵巢切除后会加剧。此外，CC治疗后注射HCG可略微改善CD98的表达。
