Dorfeshan Parvin, Ghaffari Novin Marefat, Salehi Mohammad, Farifteh Fatane
Department of Social Medicine, Faculty of Medicine, Jundishapure University of Medical Sciences, Ahvaz, Iran.
Department of Biology and Anatomical Sciences, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Int J Reprod Biomed. 2019 Jul 29;17(6):405-412. doi: 10.18502/ijrm.v17i6.4812. eCollection 2019 Jun.
The expression of miR-302 over the period of early embryogenesis could possibly regulate the maternal transcript clearance. Zygotic transcription activation is mostly related to maternal messages degradation.
In this study, the effects of in-vitro maturation technique (IVM) on the expression of miR-302 in human embryo produced from immature and mature human oocytes (matured in vitro and in vivo, before sperm exposure) obtained from females under gonadotrophin therapy were evaluated for assisted reproduction.
Immature oocytes were cultured in vitro. The injection of oocytes-producing polar bodies was given using fresh sperm. Then, the embryo quality score was assessed in the IVM group compared with the control group. In both the groups, embryos with normal morphology were included in the molecular study. Only one blastomere was removed from three-day embryos and then the embryos were frozen. The expression of miR-302 in embryos was measured through quantitative real-time polymerase chain reaction.
Our data showed a significant reduction of miR-302 expression in the IVM group vs. the control group (p = 0.02). The embryo quality score showed a significant difference between the two groups (p = 0.01).
The present study demonstrated that the IVM process had a negative effect on the expression level of miR-302 in human pre-implantation embryos. Considering the major role of expression miR-302, a reduced potential in miR-302 expression could be related to a decrease in the early embryonic development.
在早期胚胎发生过程中，miR-302的表达可能调控母体转录本的清除。合子转录激活大多与母体信息的降解有关。
在本研究中，评估体外成熟技术（IVM）对接受促性腺激素治疗的女性所获得的未成熟和成熟人类卵母细胞（在体外和体内成熟，在精子接触之前）产生的人类胚胎中miR-302表达的影响，用于辅助生殖。
未成熟卵母细胞进行体外培养。使用新鲜精子对产生极体的卵母细胞进行注射。然后，将IVM组与对照组的胚胎质量评分进行比较。在两组中，形态正常的胚胎纳入分子研究。仅从三天胚胎中取出一个卵裂球，然后将胚胎冷冻。通过定量实时聚合酶链反应测量胚胎中miR-302的表达。
我们的数据显示，与对照组相比，IVM组中miR-302的表达显著降低（p = 0.02）。两组之间的胚胎质量评分存在显著差异（p = 0.01）。
本研究表明，IVM过程对人类植入前胚胎中miR-302的表达水平有负面影响。考虑到miR-302表达的主要作用，miR-302表达潜力的降低可能与早期胚胎发育的减少有关。
