Carvalho Maria Cristina, Zuolo Mario Luis, Arruda-Vasconcelos Rodrigo, Marinho Ariane Cássia Salustiano, Louzada Lidiane Mendes, Francisco Priscila Amanda, Pecorari Vanessa Galego Arias, Gomes Brenda Paula Figueiredo de Almeida
Department of Restorative Dentistry, Piracicaba Dental School, Universidade Estadual de Campinas - Unicamp, São Paulo, Brazil.
Braz Oral Res. 2019;33:e021. doi: 10.1590/1807-3107bor-2019.vol33.0021. Epub 2019 May 16.
This study investigated the effectiveness of XP-Endo Finisher (XPF) associated with XP-Endo Shaper (XPS) or Reciproc Blue (RB) files in reducing bacterial load in oval-shaped root canals (RC) during chemomechanical preparation (CMP) using 0.9% saline solution (NaCl) or 2.5% sodium hypochlorite (NaOCl). Eighty mandibular incisors with single oval-shaped RC were contaminated with Enterococcus faecalis. The teeth were randomly assigned to eight experimental groups (n = 10) according to the CMP, as follows: G1: XPS, G2: XPS + XPF, G3: RB, and G4: RB + XPF. CMP was performed with NaCl or NaOCl. The reduction of bacterial load was assessed by colony-forming unit count before (S1) and after (S2) CMP. Data normality was verified by using Shapiro-Wilk test. ANOVA, Tukey's test, and Bonferroni post-hoc test were used at a 5% significance level. Culturable bacteria were present in all S1 samples (p>0.05). All instrumentation techniques were effective in reducing bacterial load, irrespective of the irrigating solution (p < 0.05). With the use of NaCl, RB was more effective than XPS (p = 0.035). With the use of NaOCl, XPS and RB presented similar effectiveness (p = 0.779). XPF enhanced the bacterial reduction of both systems tested (p < 0.05). The use of NaOCl improved the CMP, irrespective of the instrumentation technique used (p < 0.05). In conclusion, XPS and RB files are effective in reducing bacterial levels in oval-shaped RC. The use of XPF as a method of agitation of the irrigating solution improved the cleaning efficiency of both file systems tested. Mechanical preparation performed with saline solution decreased culturable bacteria from the root canal, but antimicrobial substances such as NaOCl should be used to achieve a significantly better disinfection.
本研究调查了XP-Endo Finisher（XPF）与XP-Endo Shaper（XPS）或Reciproc Blue（RB）锉联合使用，在使用0.9%生理盐水（NaCl）或2.5%次氯酸钠（NaOCl）进行化学机械预备（CMP）期间，降低椭圆形根管（RC）内细菌载量的效果。80颗具有单个椭圆形根管的下颌切牙被粪肠球菌污染。根据CMP方法，将牙齿随机分为8个实验组（n = 10），如下：G1：XPS，G2：XPS + XPF，G3：RB，以及G4：RB + XPF。使用NaCl或NaOCl进行CMP。通过CMP前（S1）和CMP后（S2）的菌落形成单位计数来评估细菌载量的降低情况。使用Shapiro-Wilk检验验证数据正态性。在5%的显著性水平下使用方差分析、Tukey检验和Bonferroni事后检验。所有S1样本中均存在可培养细菌（p>0.05）。无论冲洗液如何，所有预备技术在降低细菌载量方面均有效（p < 0.05）。使用NaCl时，RB比XPS更有效（p = 0.035）。使用NaOCl时，XPS和RB的效果相似（p = 0.779）。XPF增强了所测试的两种系统的细菌减少效果（p < 0.05）。无论使用何种预备技术，NaOCl的使用均改善了CMP（p < 0.05）。总之，XPS和RB锉在降低椭圆形根管内细菌水平方面是有效的。使用XPF作为冲洗液搅拌方法提高了所测试的两种锉系统的清洁效率。用生理盐水进行机械预备可减少根管内的可培养细菌，但应使用抗菌物质如NaOCl以实现明显更好的消毒效果。
