School of Dentistry and Oral Health Griffith University, Gold Coast, Queensland, Australia; Department of Dentistry, Pontifícia Universidade Católica de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil.
Laboratory of Oral Microbiology and Anaerobes, Department of Microbiology, Institute of Biological Sciences, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil.
J Endod. 2020 Sep;46(9):1228-1234. doi: 10.1016/j.joen.2020.06.007. Epub 2020 Jul 9.
This randomized clinical study compared the in vivo antibacterial efficacy of Reciproc Blue (RB), XP-endo Shaper (XP-S), and XP-endo Shaper associated with XP-endo Finisher (XP-F) systems in infected oval-shaped root canals with primary apical periodontitis.
In this study, 28 human teeth with a single root and a single canal were randomly assigned to 2 groups according to the instrumentation technique: group 1, RB (n = 14) and group 2, XP-endo (XP-S and XP-F, n = 14). The single-rooted teeth were prepared by reciprocating and rotary nickel-titanium instruments with 5.25% sodium hypochlorite irrigation. Samples were collected from the canal at the baseline (S1), after chemomechanical preparation (S2), and after XP-F instrumentation (S3). The DNA extracts were subjected to quantitative analysis for total bacterial counts by quantitative real-time polymerase chain reaction. The data were analyzed using the analysis of variance test, and the level of significance was set at 5%.
All samples tested positive for the presence of bacteria at baseline, and the bacterial counts substantially reduced after treatment procedures (P < .01). The results showed no statistical difference between RB and XP-S instrumentation with respect to the bacterial reduction (P > .05). A marked bacterial reduction was observed after the use of the XP-F instrument (P < .01).
The XP-S and RB systems sharply reduced the bacterial load in oval-shaped root canals with primary apical periodontitis. XP-F used as a supplementary instrument to chemomechanical preparation promoted a significantly higher bacterial reduction.
本随机临床研究比较了 Reciproc Blue（RB）、XP-endo Shaper（XP-S）和 XP-endo Shaper 联合 XP-endo Finisher（XP-F）系统在伴有原发性根尖周炎的感染卵圆形根管中的体内抗菌疗效。
在这项研究中，28 颗具有单根单管的人牙根据器械技术随机分为 2 组：组 1，RB（n=14）和组 2，XP-endo（XP-S 和 XP-F，n=14）。单根牙用往复和旋转镍钛器械和 5.25%次氯酸钠冲洗进行预备。在基线（S1）、化学机械预备后（S2）和 XP-F 器械后（S3）从根管中采集样本。通过定量实时聚合酶链反应对 DNA 提取物进行总细菌计数的定量分析。使用方差分析检验对数据进行分析，显著性水平设置为 5%。
所有样本基线时均检测出细菌存在，且治疗后细菌计数显著降低（P<.01）。与 XP-S 器械相比，RB 器械在细菌减少方面无统计学差异（P>.05）。使用 XP-F 器械后观察到明显的细菌减少（P<.01）。
XP-S 和 RB 系统可显著减少伴有原发性根尖周炎的卵圆形根管中的细菌负荷。XP-F 用作化学机械预备的补充器械可显著提高细菌减少率。
