超声尖与最终冲洗方案联合使用在清除长椭圆形根管和牙本质小管中的生物膜方面是否有效？

Are ultrasonic tips associated with final irrigation protocols effective in removing biofilms in long oval canals and dentinal tubules?

作者信息

Conti Leticia Citelli, Costa Vanessa Abreu Sanches Marques, Limoeiro Ana Grasiela, Coelho Jéssica de Almeida, Cuéllar Maricel Rosario Cardenas, Alcalde Murilo Priori, da Silva Guilherme Ferreira, de Andrade Flaviana Bombarda, Duarte Marco Antonio Hungaro, Vivan Rodrigo Ricci

机构信息

Department of Restorative Dentistry, Dental Materials, and Endodontics, Bauru School of Dentistry, University of São Paulo, Rua Siqueira Campos, 180, Centro, Vitória da Conquista, Bauru, São Paulo, BA, ZIP: 45.000-455, Brazil.

出版信息

Clin Oral Investig. 2025 Jan 25;29(1):90. doi: 10.1007/s00784-025-06166-y.

DOI:10.1007/s00784-025-06166-y

PMID:39862315

Abstract

OBJECTIVE

This study investigated the associations among endodontic instruments, ultrasonic tips and various final irrigation protocols for removing intracanal and intratubular biofilms in long oval canals.

METHODOLOGY

One hundred mandibular premolars inoculated with Enterococcus faecalis were divided into two groups: the control group (CG: n = 10), which received no treatment; and the test groups (n = 30), which included saline (SS), sodium hypochlorite (2.5% NaOCl) and chlorhexidine (2% CHX). The samples were prepared with Reciproc Blue 25/0.08, Flatsonic, Clearsonic and Reciproc Blue 40/0.06. Each test group was divided into three subgroups (n = 10): conventional irrigation (CI) with syringes and needles, passive ultrasonic irrigation (PUI) and continuous ultrasonic irrigation (CUI) with an Irrisonic tip. The root canals were examined using confocal laser scanning microscopy. Mann‒Whitney and Kruskal‒Wallis tests followed by Dunn post hoc tests (p < 0.05) were used for statistical analysis.

RESULTS

NaOCl and CHX were effective for intracanal and intratubular decontamination. Saline showed significant decontamination in the intratubular areas when CUI was used. The CI method was the least effective for intracanal decontamination.

CONCLUSION

The use of Flatsonic and Clearsonic ultrasonic tips is promising for root canal disinfection.

CLINICAL RELEVANCE

The disinfection techniques in endodontic treatment focus on the removal of biofilms from long oval canals, which is crucial for effective cleaning during root canal procedures.

摘要

目的

本研究调查了根管器械、超声尖以及各种最终冲洗方案在去除长椭圆形根管内和管内生物膜方面的相关性。

方法

将100颗接种粪肠球菌的下颌前磨牙分为两组：对照组（CG：n = 10），不接受任何治疗；试验组（n = 30），包括生理盐水（SS）、次氯酸钠（2.5% NaOCl）和洗必泰（2% CHX）。样本使用Reciproc Blue 25/0.08、Flatsonic、Clearsonic和Reciproc Blue 40/0.06进行制备。每个试验组再分为三个亚组（n = 10）：用注射器和针头进行的传统冲洗（CI）、被动超声冲洗（PUI）以及使用Irrisonic尖进行的连续超声冲洗（CUI）。使用共聚焦激光扫描显微镜检查根管。采用Mann-Whitney和Kruskal-Wallis检验，随后进行Dunn事后检验（p < 0.05）进行统计分析。