Department of Reproductive, The Second Affiliated Hospital of Kunming Medical University, Kunming Yunnan, China.
Department of Obstetrics and Gynecology, The First Affiliated Hospital of Xiamen University, Xiamen Fujian, China.
Bosn J Basic Med Sci. 2020 Feb 5;20(1):125-130. doi: 10.17305/bjbms.2019.4352.
No study examined the frequency of human papillomavirus (HPV) genotypes by surface plasmon resonance (SPR) in Southwest China. This was a cross-sectional survey (The Second Affiliated Hospital of Kunming Medical University, 10/2010 to 12/2011) in 150 patients who were hospitalized or volunteered for cervical cancer (CC) screening. A HPV typing kit was used to detect 24 types of HPV by the SPR technique. The HPV-positive rate was 34.8% in women with normal cytology and 92.9% in women with CC. The frequency of HPV16 increased from 9.4% for women with normal cytology to 28.9% for cervical intraepithelial neoplasia (CIN)1, 41.4% for CIN2, 54.1% for CIN3, and 71.4% for CC (p < 0.001). The frequency of HPV18 increased from 0% for women with normal cytology to 2.6% for CIN1, 3.4% for CIN2, 5.4% for CIN3, and 21.4% for CC (p = 0.03). HPV40 was only found in one patient with CC (p = 0.04). There was no relation between HPV genotype and women's age. In Kunming (Southwest China), the frequency of HPV infection was 74.0% among women who underwent CC screening. HPV16 and HPV18 were the two most frequent genotypes. SPR could be of value for the screening of HPV infection.
在中国西南地区，尚无研究采用表面等离子共振（SPR）技术检测人乳头瘤病毒（HPV）基因型的频率。这是一项横断面研究（昆明医科大学第二附属医院，2010 年 10 月至 2011 年 12 月），共纳入 150 例因宫颈癌（CC）住院或自愿接受筛查的患者。采用 HPV 基因分型试剂盒通过 SPR 技术检测 24 种 HPV 型别。细胞学正常者 HPV 阳性率为 34.8%，CC 患者为 92.9%。HPV16 的频率从细胞学正常者的 9.4%上升至 CIN1 的 28.9%、CIN2 的 41.4%、CIN3 的 54.1%和 CC 的 71.4%（p＜0.001）。HPV18 的频率从细胞学正常者的 0%上升至 CIN1 的 2.6%、CIN2 的 3.4%、CIN3 的 5.4%和 CC 的 21.4%（p=0.03）。HPV40 仅在 1 例 CC 患者中检出（p=0.04）。HPV 基因型与患者年龄无关。在昆明（中国西南地区），接受 CC 筛查的女性 HPV 感染率为 74.0%。HPV16 和 HPV18 是最常见的两种基因型。SPR 可能有助于 HPV 感染的筛查。
