Faculty of Environmental Science and Engineering & Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming 650500, People's Republic of China; Department of Gynecology, The First People's Hospital of Yunnan Province Kunming, 630030, People's Republic of China.
College of Veterinary Medicine, South China Agricultural University, Guangzhou, 510642, People's Republic of China.
J Infect Public Health. 2018 Jan-Feb;11(1):105-110. doi: 10.1016/j.jiph.2017.06.012. Epub 2017 Jul 8.
This study was designed to explore baseline data about the prevalence and distribution of human papillomavirus (HPV) genotypes among Chinese women who had cervical intraepithelial lesions and cervical cancer. A total of 511 patients were recruited, and biopsy samples were collected from these patients. Polymerase chain reaction was used to detect HPV-positive samples, and the HPV GenoArray kit was used for genotyping. A total of 23 genotypes were detected, including 13 that were high risk-HPV (HR-HPV), 3 that were potential high risk-HPV (PHR-HPV) and 7 that were low risk-HPV (LR-HPV). The prevalence rates of HPV infection in Han women diagnosed with cervical intraepithelial lesions (CIN) 1, 2, and 3 and squamous cell carcinoma (SCC) were 98.30%, 97.56, 100% and 90%, respectively. The HPV-positive cases in women of other ethnicities diagnosed with CIN1, CIN2, and CIN3 and SCC were 95%, 90.91%, 88.23% and 83.33%, respectively. The most frequent genotypes in both ethnic groups were HPV-16, 52, and 58. LR-HPV was detected in SCC lesions in the non-Han ethnic group. In the Han ethnic group, the LR-HPV genotype was mostly restricted to CIN1 lesions. Furthermore, we found a high prevalence of PHR-HPV-81 in SCC lesions among Han women. Ethnic background, smoking, sex at an early age, unprotected sex, use of contraceptives, and the withdrawal method were found to be significantly associated with HPV infection. In conclusion, this study explores epidemiological data regarding the prevalence of HPV and the genotype distribution in patients with SCC and CIN lesions in Yunnan Province, China.
本研究旨在探讨中国宫颈上皮内瘤变（CIN）和宫颈癌患者中 HPV 基因型的流行率和分布情况的基线数据。共招募了 511 名患者，并从这些患者中采集活检样本。采用聚合酶链反应（PCR）检测 HPV 阳性样本，使用 HPV GenoArray 试剂盒进行基因分型。共检测到 23 种基因型，包括 13 种高危型 HPV（HR-HPV）、3 种潜在高危型 HPV（PHR-HPV）和 7 种低危型 HPV（LR-HPV）。诊断为 CIN1、2、3 和鳞状细胞癌（SCC）的汉族妇女 HPV 感染率分别为 98.30%、97.56%、100%和 90%。诊断为 CIN1、2 和 3 的其他种族妇女 HPV 阳性病例分别为 95%、90.91%、88.23%和 83.33%。两组中最常见的基因型均为 HPV-16、52 和 58。LR-HPV 在非汉族 SCC 病变中检测到。在汉族中，LR-HPV 基因型主要局限于 CIN1 病变。此外，我们发现汉族 SCC 病变中 PHR-HPV-81 的流行率较高。研究发现，种族背景、吸烟、初次性行为年龄较小、无保护性行为、使用避孕药具和退出方法与 HPV 感染显著相关。综上所述，本研究探讨了中国云南省 SCC 和 CIN 病变患者中 HPV 流行率和基因型分布的流行病学数据。
