Department of Medicine, Division of Infectious Diseases, University of British Columbia, Vancouver, BC, Canada; Laboratorio de Nanobiomateriales, Facultad de Estomatología, Universidad Autónoma de San Luis Potosí, Mexico.
Division of Medical Microbiology, St. Paul's Hospital, Providence Health Care, Vancouver, BC, Canada; Department of Pathology and Laboratory Medicine, University of British Columbia, Vancouver, BC, Canada.
Nanomedicine. 2019 Nov;22:102093. doi: 10.1016/j.nano.2019.102093. Epub 2019 Sep 13.
Plasmonic photothermal therapy (PPTT) has been used as an alternative to chemotherapy for the elimination of resistant microorganisms; however, its in situ evaluation has not been well studied. In the present study, we assessed the antimicrobial activity of a chitosan-based hydrogel embedded with gold nanorods (Ch/AuNRs) using a low power infrared diode laser. The antibacterial activity was measured in both Gram-positive and -negative strains, including clinical isolates of multidrug-resistant pathogens. The cytotoxic effect, cellular proliferation, and the expression of the pro-inflammatory (IL-6 and TNF-α) and anti-inflammatory (IL-10) cytokines were quantified in a murine model of macrophages. Results showed a potent antimicrobial activity of the Ch/AuNRs with MICs ≤4 μg/mL, very low cytotoxicity with cell viability above 80%, and the macrophage proliferation was not affected for a period of 48 h. These results suggest that our Ch/AuNR-embedded hydrogel could be an option to locally control chronic nosocomial infections using PPTT.
等离子体光热疗法 (PPTT) 已被用作替代化疗来消除耐药微生物的方法；然而，其原位评估尚未得到充分研究。在本研究中，我们使用低功率红外二极管激光评估了负载金纳米棒的壳聚糖水凝胶（Ch/AuNRs）的抗菌活性。对革兰氏阳性和革兰氏阴性菌株（包括多药耐药病原体的临床分离株）进行了抗菌活性测量。在巨噬细胞的小鼠模型中，定量了细胞毒性、细胞增殖以及促炎（IL-6 和 TNF-α）和抗炎（IL-10）细胞因子的表达。结果表明，Ch/AuNRs 具有强大的抗菌活性，MICs≤4μg/mL，细胞活力高于 80%，细胞毒性非常低，并且巨噬细胞增殖在 48 小时内不受影响。这些结果表明，我们的负载金纳米棒的壳聚糖水凝胶可以作为一种选择，用于使用 PPTT 局部控制慢性医院获得性感染。
