Salem Dalia A, Stetler-Stevenson Maryalice
Laboratory of Pathology, CCR, NCI, NIH, Bethesda, MD, USA.
Clinical Pathology Department, Faculty of Medicine, Mansoura University, Mansoura, Egypt.
Methods Mol Biol. 2019;2032:311-321. doi: 10.1007/978-1-4939-9650-6_17.
Flow-cytometric demonstration of the typical chronic lymphocytic leukemia (CLL) immunophenotype is vital for diagnosis. CLL has a characteristic immunophenotype, expressing CD5, CD19, dim CD20, dim CD22, CD23, bright CD43, dim CD45, dim to negative CD79b, dim CD81, CD200, and dim monoclonal surface immunoglobulin. This characteristic immunophenotype allows a definitive diagnosis and the ruling out of another leukemia or lymphoma. Flow cytometry also provides important prognostic information and accurate assessment of response to therapy. Here we describe optimal specimen collection, red cell lysis, appropriate panel, cell staining, acquisition on a flow cytometer, and analysis for CLL specimens.
通过流式细胞术证明典型慢性淋巴细胞白血病（CLL）免疫表型对诊断至关重要。CLL具有特征性免疫表型，表达CD5、CD19、弱阳性CD20、弱阳性CD22、CD23、强阳性CD43、弱阳性CD45、弱阳性至阴性CD79b、弱阳性CD81、CD200以及弱阳性单克隆表面免疫球蛋白。这种特征性免疫表型有助于明确诊断并排除其他白血病或淋巴瘤。流式细胞术还可提供重要的预后信息以及对治疗反应的准确评估。在此，我们描述CLL标本的最佳样本采集、红细胞裂解、合适的抗体组合、细胞染色、流式细胞仪采集及分析方法。
