Ghorbani Mohammad Mehdi, Farazmandfar Touraj, Nasirikenari Mehrab, Abediankenari Saeid, Meamarian Ali, Shahbazi Majid
Medical Cellular and Molecular Research Center, Golestan University of Medical Sciences, Gorgan, Iran.
Pasteur Institute of Amol, Amol, Iran.
Iran J Allergy Asthma Immunol. 2019 Jun 8;18(3):300-309. doi: 10.18502/ijaai.v18i3.1123.
Multiple sclerosis (MS) is an autoimmune disease that affects the central nervous system.MS creates a wide range of symptoms with lifelong debilitating consequences. The hallmark of the disease is the inflammation of the nervous system, which can lead to damage to the nerve tissue and loss of function of the neurons. IL-17 has a prominent role in the beginning of inflammatory reactions. Here, we analyzed a mouse model developed using anti-myelin antibodies. This mouse model mimics many symptoms of MS in humans. C57BL/6 mice were randomly divided into five groups. Mice were immunized subcutaneously with 50 μg, 100 μg, 150 μg and 200 μg myelin oligodendrocyte glycoprotein in complete Freund's adjuvant containing 4 mg/Ml Mycobacterium tuberculosis and two injections of 800 ng of pertussis toxin intraperitoneally, on day 0 and 2 post immunization. Serum level of IL-17 was measured, inflammation and demyelination of brain tissue were also evaluated. Mice with experimental autoimmune encephalomyelitis demonstrated inflammatory cell accumulation, different degrees of demyelination in the brain, and rising levels of serum IL-17 depending on the dose of the anti-myelin antibody. Our study demonstrates that level of IL-17 production is directly associated with inflammation and demyelination. In addition, different degrees of experimental autoimmune encephalomyelitis in mice can be utilized to test a wide range of therapeutic interventions for MS treatment.
多发性硬化症（MS）是一种影响中枢神经系统的自身免疫性疾病。MS会引发一系列症状，并带来终身的衰弱后果。该疾病的标志是神经系统炎症，这会导致神经组织受损和神经元功能丧失。白细胞介素-17（IL-17）在炎症反应的起始阶段发挥着重要作用。在此，我们分析了一种使用抗髓鞘抗体构建的小鼠模型。这种小鼠模型模拟了人类MS的许多症状。将C57BL/6小鼠随机分为五组。在免疫接种的第0天和第2天，小鼠皮下注射含4mg/Ml结核分枝杆菌的完全弗氏佐剂中的50μg、100μg、150μg和200μg髓鞘少突胶质细胞糖蛋白，并腹腔注射两次800ng百日咳毒素。检测血清IL-17水平，同时评估脑组织的炎症和脱髓鞘情况。患有实验性自身免疫性脑脊髓炎的小鼠表现出炎症细胞聚集、大脑不同程度的脱髓鞘，并且血清IL-17水平会根据抗髓鞘抗体的剂量而升高。我们的研究表明，IL-17的产生水平与炎症和脱髓鞘直接相关。此外，小鼠不同程度的实验性自身免疫性脑脊髓炎可用于测试多种针对MS治疗的干预措施。
