Harris-Warrick R M, Hammond C, Paupardin-Tritsch D, Homburger V, Rouot B, Bockaert J, Gerschenfeld H M
Laboratoire de Neurobiologie, Ecole Normale Supérieure, Paris, France.
Neuron. 1988 Mar;1(1):27-32. doi: 10.1016/0896-6273(88)90206-1.
Dopamine induces a decrease in voltage-dependent Ca2+ current in identified neurons of the snail H. aspersa. This effect is blocked by intracellular injection of activated B. pertussis toxin and of an affinity-purified antibody against the alpha subunit of bovine Go protein. The dopamine effect is mimicked by intracellular injection of mammalian alpha o. In snail nervous tissue, pertussis toxin ADP-ribosylates a single protein band on SDS gels, and this band is recognized in immunoblots by the anti-alpha o antibody. We propose that this is a 40 kd alpha subunit of a molluscan G protein immunologically related to alpha o and that it mediates the effect of dopamine on Ca2+ currents in identified snail neurons.
多巴胺可使蜗牛（Helix aspersa）特定神经元中电压依赖性Ca2+电流降低。细胞内注射活化的百日咳毒素以及针对牛Go蛋白α亚基的亲和纯化抗体可阻断此效应。细胞内注射哺乳动物αo可模拟多巴胺的效应。在蜗牛神经组织中，百日咳毒素可使SDS凝胶上的一条单一蛋白带发生ADP核糖基化，并且这条带在免疫印迹中可被抗αo抗体识别。我们推测这是一种与αo免疫相关的软体动物G蛋白的40 kdα亚基，它介导多巴胺对特定蜗牛神经元Ca2+电流的作用。
