Chernigova Svetlana Vladimirovna, Chernigov Yury Vladimirovich, Vatnikov Yury Anatolyevich, Kulikov Evgeny Vladimirovich, Popova Irina Anatolyevna, Shirmanov Vyacheslav Ivanovich, Molchanova Mariya Andreyevna, Likhacheva Irina Fedorovna, Voronina Yuliya Yuryevna, Lukina Darya Mikhaylovna
Department of Veterinary Medicine, Omsk State Agrarian University Named After P. A. Stolypin, Omsk, Russia.
Department of Livestock, Omsk Agricultural Research Center, Omsk, Russia.
Vet World. 2019 Jul;12(7):932-937. doi: 10.14202/vetworld.2019.932-937. Epub 2019 Jul 2.
In this study, we identified characteristics of systemic inflammation associated with surgical sepsis in animals. We evaluated the role of purine metabolism, functionally associated lipoperoxidation processes of membrane structures, and the antioxidant system in the development of surgical sepsis in dogs.
Dogs with a provisional exclusion of sepsis were included in the study. The control group (Group 1) included clinically healthy dogs (n=5), and medium-breed dogs with systemic inflammation response syndrome (n=30) were categorized in the experimental group (Group 2). Along with hemogram and biochemical analysis, we determined the amount of malondialdehyde, glutathione, superoxide dismutase, catalase, glutathione reductase, and glucose-6-phosphate dehydrogenase on the 1 and 14 day of the study. Treatment included a thorough reorganization of the septic focus, followed by antibacterial therapy. Sick animals were injected with a drug (dexamethasone) that suppresses the synthesis and inhibits the action of inflammatory mediators. Decompensation of the functions of organs and systems was carried out using symptomatic therapy.
We found that enhanced lipid peroxidation of unsaturated fatty acids of membrane structures stimulates the generalization of inflammatory process, as evidenced by the significant deviation from the physiologically normal values of lipid peroxidation, C-reactive protein, blood cell count, etc. The course of systemic inflammation associated with surgical sepsis in animals can be attributed to several consistently developing processes that function as a result of increased purine mononucleotide catabolism, peroxide compound formation, and their excessive breakdown in reactions associated with the consumption of glutathione due to the insufficient recovery of glutathione disulfide.
The amount of uric acid, glycosaminoglycans, hyaluronic acid in blood plasma, and the content of malondialdehyde, glutathione, and glutathione reductase in erythrocytes should be considered when assessing the severity of the systemic inflammatory process. The increased glutathione requirement in dogs with surgical sepsis requires intervention with pharmacological agents, and further research is needed in this aspect.
在本研究中，我们确定了与动物手术性脓毒症相关的全身炎症特征。我们评估了嘌呤代谢、膜结构功能相关的脂质过氧化过程以及抗氧化系统在犬手术性脓毒症发展中的作用。
将初步排除脓毒症的犬纳入研究。对照组（第1组）包括临床健康的犬（n = 5），实验组（第2组）为患有全身炎症反应综合征的中型犬（n = 30）。除血常规和生化分析外，我们在研究的第1天和第14天测定了丙二醛、谷胱甘肽、超氧化物歧化酶、过氧化氢酶、谷胱甘肽还原酶和葡萄糖-6-磷酸脱氢酶的含量。治疗包括对感染灶进行彻底清理，随后进行抗菌治疗。患病动物注射一种抑制炎症介质合成并抑制其作用的药物（地塞米松）。使用对症治疗对器官和系统功能失代偿进行处理。
我们发现膜结构不饱和脂肪酸的脂质过氧化增强会刺激炎症过程的扩散，脂质过氧化、C反应蛋白、血细胞计数等生理正常数值出现显著偏差即可证明。与动物手术性脓毒症相关的全身炎症过程可归因于几个持续发展的过程，这些过程是由于嘌呤单核苷酸分解代谢增加、过氧化物化合物形成以及它们在与谷胱甘肽消耗相关的反应中过度分解（由于谷胱甘肽二硫化物恢复不足）而产生的。
在评估全身炎症过程的严重程度时，应考虑血浆中尿酸、糖胺聚糖、透明质酸的含量以及红细胞中丙二醛、谷胱甘肽和谷胱甘肽还原酶的含量。手术性脓毒症犬对谷胱甘肽的需求增加需要使用药物进行干预，这方面需要进一步研究。
