Michanie S, Bryan F L, Alvarez P, Olivo A B, Paniagua A
Pan American Health Organization, Pan American Zoonoses Center, Buenos Aires, Argentina.
Int J Food Microbiol. 1988 Oct;7(2):123-34. doi: 10.1016/0168-1605(88)90005-0.
Hazard analysis of food preparation practices were conducted in four households and eleven others were visited to survey both food preparation practices and environmental conditions. Households selected had members who were suffering from either diarrhea of unknown etiology or alleged typhoid fever. Hazard analyses and sanitary surveys included gathering data on time-temperature exposures of foods, collecting samples of food and drinking water, sampling sewage or drains, and obtaining stool specimens from persons with diarrhea and from family controls. Food samples were tested for aerobic mesophilic colony counts and common foodborne pathogens; specimens were tested for Salmonella, Shigella, Campylobacter and Yersinia. Campylobacter was isolated from two persons purported to have diarrhea, but neither Salmonella, Shigella nor Yersinia were recovered from alleged cases or controls. Salmonella agona was recovered from a latrine. Most foods were cooked to internal temperatures to or near to boiling. Those not promptly eaten were held at ambient room or outside temperatures until a subsequent meal, until a family member returned home, or until lunch time when taken to the fields. During these intervals, microorganisms multiplied and mesophilic aerobic organisms increased often reaching 10(8)/g or greater before consumption. None of these foods were reheated before eating. Bacillus cereus was isolated from 4 of 10 samples; one sample of 'moro' (beans and rice) exceeded 10(6)/g, two other samples exceeded 10(3)/g. Staphylococcus aureus was isolated from 7 of 14 samples, one exceeded 10(5)/g. Fecal coliforms were isolated from 8 of 14 food samples, five exceeded 10(5)/g. Neither Salmonella nor Shigella were isolated from any food, the community water supplies or from vessels of water within houses. Fecal coliform counts of water were less than 3/ml, except one sample from a clay vessel (9/ml). Risks associated with cooked foods which were not promptly eaten appeared to be greater than that associated with water.
对四个家庭的食品制备做法进行了危害分析,并走访了另外十一个家庭,以调查食品制备做法和环境状况。所挑选的家庭中有成员患有病因不明的腹泻或疑似伤寒热。危害分析和卫生调查包括收集食品的时间-温度暴露数据、采集食品和饮用水样本、对污水或排水管道进行采样,以及从腹泻患者和家庭对照中获取粪便样本。对食品样本进行需氧嗜温菌菌落计数和常见食源性病原体检测;对样本进行沙门氏菌、志贺氏菌、弯曲杆菌和耶尔森氏菌检测。从两名疑似腹泻患者中分离出弯曲杆菌,但在疑似病例或对照中均未检出沙门氏菌、志贺氏菌或耶尔森氏菌。从一个厕所中检出阿哥纳沙门氏菌。大多数食品煮至内部温度达到或接近沸腾。那些未立即食用的食品在室温或室外温度下存放,直到下一顿饭、直到家庭成员回家,或者直到午餐时间带到田间。在这些间隔期间,微生物繁殖,嗜温需氧生物数量经常增加,在食用前通常达到10⁸/g或更高。这些食品在食用前均未重新加热。从10个样本中的4个分离出蜡样芽孢杆菌;一份“莫罗”(豆类和米饭)样本超过10⁶/g,另外两份样本超过10³/g。从14个样本中的7个分离出金黄色葡萄球菌,一份超过10⁵/g。从14个食品样本中的8个分离出粪大肠菌群,5个超过10⁵/g。在任何食品、社区供水或房屋内的水容器中均未分离出沙门氏菌或志贺氏菌。水中粪大肠菌群计数除一份来自陶制容器的样本(9/ml)外均低于3/ml。未立即食用的熟食相关风险似乎大于与水相关的风险。