Syakalima Michelo, Ramatla Tsepo, Lubanza Ngoma
Department of Animal Health, School of Agriculture, Faculty of Natural and Agricultural Sciences, Mafikeng Campus, North-West University, Private Bag X2046, Mmabatho 2735, South Africa.
Vet World. 2019 Jul;12(7):1066-1069. doi: 10.14202/vetworld.2019.1066-1069. Epub 2019 Jul 18.
Pigeon feces are increasingly being implicated in the spread of bacterial pathogens such as , , , , and . Fungi are rarely investigated except for that has emerged as an important pathogen in old people and immunosuppressed patients. This study investigated fungi in pigeon feces collected from Mafikeng, the North West Province of South Africa.
Freshly dropped feces were collected and enriched in phosphate-buffered saline overnight at 48°C and then subcultured on Sabouraud's dextrose agar and incubated at 48°C for 2 weeks observing any fungal growth from day 2. The growths were picked up, DNA extracted, and polymerase chain reaction was done using the internal transcribed spacer primers.
Fungi isolated included: (), ( and ), spp., and ( and ). Most of these isolates are known opportunistic pathogens and have been isolated in clinical conditions elsewhere. Other isolates such as , , , , and were not found to be of any human health significance.
We, therefore, concluded that the presence of these opportunistic pathogens is a significant human health risk, especially in the face of the HIV/AIDS pandemic that results in immunosuppression.
鸽粪越来越多地被认为与诸如[具体细菌名称1]、[具体细菌名称2]、[具体细菌名称3]、[具体细菌名称4]和[具体细菌名称5]等细菌病原体的传播有关。除了[某种真菌名称]已成为老年人和免疫抑制患者中的重要病原体外，真菌很少被研究。本研究调查了从南非西北省马菲肯收集的鸽粪中的真菌。
收集新鲜粪便，在48°C下于磷酸盐缓冲盐水中过夜富集，然后接种于沙氏葡萄糖琼脂培养基上，在48°C下培养2周，从第2天开始观察是否有真菌生长。挑取生长物，提取DNA，并使用内转录间隔区引物进行聚合酶链反应。
分离出的真菌包括：[真菌名称1]（[具体描述1]）、[真菌名称2]（[具体描述2]和[具体描述3]）、[真菌名称3]属、以及[真菌名称4]（[具体描述4]和[具体描述5]）。这些分离株大多是已知的机会性病原体，已在其他地方的临床病例中分离得到。其他分离株如[具体真菌名称5]、[具体真菌名称6]、[具体真菌名称7]、[具体真菌名称8]和[具体真菌名称9]未发现对人类健康有任何意义。
因此，我们得出结论，这些机会性病原体的存在对人类健康构成重大风险，尤其是在面临导致免疫抑制的艾滋病毒/艾滋病大流行的情况下。
