Laboratory of Physical and Analytical Electrochemistry , École Polytechnique Fédérale de Lausanne (EPFL) , Valais Wallis, Rue de l'Industrie 17 , CH-1950 Sion , Switzerland.
Institute of Chemical Sciences and Engineering , École Polytechnique Fédérale de Lausanne, EPFL , CH-1015 Lausanne , Switzerland.
Anal Chem. 2019 Oct 15;91(20):12900-12908. doi: 10.1021/acs.analchem.9b02819. Epub 2019 Sep 26.
A noninvasive electrochemical melanoma detection approach based on using adhesive tapes for collecting and fixing cells from a suspicious skin area and transferring the cells into a scanning electrochemical microscope (SECM) is presented. The adhesive layer collects the cells reproducibly and keeps them well adhered on the tape during experiments in an electrolyte solution. A melanoma biomarker, here the intracellular enzyme tyrosinase (TYR), was imaged on the tape-collected cells without further cell lysing using antibodies that were labeled with horseradish peroxidase (HRP). The HRP labels catalyzed the oxidation of a dissolved redox-active species, which was detected at a soft microelectrode, gently brushed in contact mode over the tape. The melanoma biomarker was first detected on tape-stripped samples with murine melanoma cells of different concentrations. Thereafter, increasing levels of TYR were recorded in cells that were collected from the skin of melanoma mouse models representing three different stages of tumor growth. Additionally, SECM results of tape-stripped different human melanoma cell lines were confirmed by previous studies based on traditionally fixed and permeabilized cells.
提出了一种基于使用胶带收集和固定可疑皮肤区域细胞并将细胞转移到扫描电化学显微镜（SECM）的无创电化学黑色素瘤检测方法。在电解质溶液中进行实验时，胶带层可重复收集细胞，并使细胞在胶带上保持良好的粘附性。使用辣根过氧化物酶（HRP）标记的抗体，无需进一步细胞裂解，即可对胶带收集的细胞上的黑色素瘤生物标志物，即细胞内酶酪氨酸酶（TYR）进行成像。HRP 标签催化溶解的氧化还原活性物质的氧化，该物质在软微电极上以接触模式轻轻刷过胶带进行检测。首先在不同浓度的鼠黑色素瘤细胞的胶带剥离样品上检测到了黑色素瘤生物标志物。此后，在代表肿瘤生长三个不同阶段的黑色素瘤小鼠模型的皮肤中收集的细胞中记录到 TYR 水平逐渐升高。此外，基于传统固定和通透细胞的先前研究证实了胶带剥离的不同人黑色素瘤细胞系的 SECM 结果。
