Department of Biomedical Science, Faculty of Health and Society, Malmö University, 205 06, Malmö, Sweden.
Biofilms - Research Center for Biointerfaces, Malmö University, 205 06, Malmö, Sweden.
Mikrochim Acta. 2020 Nov 13;187(12):656. doi: 10.1007/s00604-020-04633-9.
Elevated amounts of reactive oxygen species (ROS) including hydrogen peroxide (HO) are observed in the epidermis in different skin disorders. Thus, epidermal sensing of HO should be useful to monitor the progression of skin pathologies. We have evaluated epidermal sensing of HO in vitro, by visualising HO permeation through the skin. Skin membranes were mounted in Franz cells, and a suspension of Prussian white microparticles was deposited on the stratum corneum face of the skin. Upon HO permeation, Prussian white was oxidised to Prussian blue, resulting in a pattern of blue dots. Comparison of skin surface images with the dot patterns revealed that about 74% of the blue dots were associated with hair shafts. The degree of the Prussian white to Prussian blue conversion strongly correlated with the reciprocal resistance of the skin membranes. Together, the results demonstrate that hair follicles are the major pathways of HO transdermal penetration. The study recommends that the development of HO monitoring on skin should aim for pathway-specific epidermal sensing, allowing micrometre resolution to detect and quantify this ROS biomarker at hair follicles.Graphical abstract.
在不同的皮肤疾病中，表皮中观察到活性氧（ROS）的含量升高，包括过氧化氢（HO）。因此，表皮对 HO 的感知应该有助于监测皮肤病变的进展。我们通过可视化 HO 通过皮肤的渗透来评估体外表皮对 HO 的感知。将皮肤膜安装在 Franz 细胞中，并将普鲁士白微颗粒悬浮液沉积在皮肤的角质层表面。HO 渗透后，普鲁士白被氧化为普鲁士蓝，导致出现蓝色斑点图案。将皮肤表面图像与点图案进行比较后发现，约 74%的蓝色斑点与毛发有关。普鲁士白到普鲁士蓝的转化程度与皮肤膜的倒数电阻强烈相关。综上所述，这些结果表明毛囊是 HO 经皮渗透的主要途径。该研究建议，HO 监测的开发应针对特定途径的表皮感知，以实现微毫米分辨率，从而在毛囊处检测和量化这种 ROS 生物标志物。
