Rajabi Parvin, Heydarpoor Mitra, Maghsoudi Ahmadreza, Mohaghegh Fatemeh, Dehghani Mobarakeh Maryam
Dept. of Pathology, Isfahan University of Medical Sciences, Isfahan, Iran.
Internal Medicine Dept., Isfahan University of Medical Sciences, Isfahan, Iran.
Iran J Pathol. 2019 Winter;14(1):52-60. doi: 10.30699/IJP.14.1.52. Epub 2018 Dec 27.
BACKGROUND & OBJECTIVE: Basal cell carcinoma (BCC) is a common skin cancer arising from the basal layer of the epidermis and its appendages. They are locally invasive, aggressive, and destructive of skin and the surrounding struc- tures. -Catenin is a multifunctional protein located to the intracellular side of the cytoplasmic membrane coded by the gene, which maps to chromosome 3p22.1. It has a critical role in cell-to-cell adhesion by linking cadherins to the actin cytoskeleton and has a central role in transcriptional regulation in the Wnt signaling pathway. We evaluated the diagnostic value of the Beta catenin immunohistochemistry marker in distinction of aggressive and non-aggressive Basal cell carcinoma.
This cross sectional and descriptive-analytical study was done on archived formalin fixed, paraffin embed- ded tissue blocks in pathology library of Al-Zahra hospital in Isfahan city. We used immunochemistry to determinate the role of -Catenin in aggressiveness in BCC with higher rate of relapse.
A total of 76 samples were evaluated in two groups (aggressive &none aggressive). The mean percentage of cytoplasmic -Catenin staining in aggressive group was more significant than the other group (sensitivity: 86.8% specificity: 81.6%, PPV: 81.5% and NPV: 86.1%) and the mean percentage of membranous β-Catenin staining in non- aggressive group were significant more than the aggressive group. Intensity of membranous staining in both groups significant less than normal epithelium.
Cytoplasmic -Catenin staining in aggressive BCC is more significant than non-aggressive subtypes, so this indicates that the use of -Catenin IHC marker maybe helpful in the diagnosis of aggressive BCC.
基底细胞癌（BCC）是一种常见的皮肤癌，起源于表皮及其附属器的基底层。它们具有局部侵袭性、攻击性，会破坏皮肤及周围组织。β-连环蛋白是一种多功能蛋白，位于细胞质膜的细胞内侧，由位于染色体3p22.1的基因编码。它通过将钙黏蛋白与肌动蛋白细胞骨架相连，在细胞间黏附中起关键作用，并且在Wnt信号通路的转录调控中起核心作用。我们评估了β-连环蛋白免疫组化标志物在鉴别侵袭性和非侵袭性基底细胞癌中的诊断价值。
本横断面描述性分析研究在伊斯法罕市扎赫拉医院病理库中存档的福尔马林固定、石蜡包埋组织块上进行。我们使用免疫化学方法来确定β-连环蛋白在复发率较高的基底细胞癌侵袭性中的作用。
共对两组（侵袭性和非侵袭性）的76个样本进行了评估。侵袭性组中细胞质β-连环蛋白染色的平均百分比比另一组更显著（敏感性：86.8%，特异性：81.6%，阳性预测值：81.5%，阴性预测值：86.1%），非侵袭性组中膜性β-连环蛋白染色的平均百分比比侵袭性组显著更高。两组中膜性染色强度均显著低于正常上皮。
侵袭性基底细胞癌中细胞质β-连环蛋白染色比非侵袭性亚型更显著，因此这表明使用β-连环蛋白免疫组化标志物可能有助于侵袭性基底细胞癌的诊断。
